Name: Anti-HELLS Antibody (CAB5831)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB5831
Availability: OutOfStock

Antibody Name:	Anti-HELLS Antibody [KO Validated]
Antibody SKU:	CAB5831
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, A549, 293T, HepG2, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VYRL VTAN TIDQ KIVE RAAA KRKL EKLI IHKN HFKG GQSG LNLS KNFL DPKE LMEL LKSR DYER EIKG SREK VISD KDLE LLLD RSDL IDQM NASG PIKE KMGI FKIL ENSE DSSP ECLF
Gene ID:	3070
Uniprot:	Q9NRZ9
Cellular Location:	Nucleus
Calculated MW:	14kDa/36-39kDa/81-97kDa
Observed MW:	120kDa

Synonyms:	HELLS, ICF4, LSH, Nbla10143, PASG, SMARCA6
Background:	This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	HELLS: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis. By concanavalin-A in peripheral blood leukocytes. Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias. Belongs to the SNF2/RAD54 helicase family. 9 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.6.1.-; EC 3.6.4.-; Helicase; Apoptosis Chromosomal Location of Human Ortholog: 10q24.2 Cellular Component: centric heterochromatin; nucleus; chromosome, pericentric region Molecular Function:protein binding; DNA binding; helicase activity; ATP binding Biological Process: mitosis; lymphocyte proliferation; transcription, DNA-dependent; methylation-dependent chromatin silencing; maintenance of DNA methylation; cell division; multicellular organismal development; centric heterochromatin formation
NCBI Summary:	This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]
UniProt Code:	Q9NRZ9
NCBI GenInfo Identifier:	74761670
NCBI Gene ID:	3070
NCBI Accession:	Q9NRZ9.1
UniProt Secondary Accession:	Q9NRZ9,Q3LID1, Q6I7N7, Q76H76, Q76H77, Q76H78, Q76H79 Q76H80, Q76H81, Q7Z397, Q7Z5X2, B2RB41,
UniProt Related Accession:	Q9NRZ9
Molecular Weight:	838
NCBI Full Name:	Lymphoid-specific helicase
NCBI Synonym Full Names:	helicase, lymphoid-specific
NCBI Official Symbol:	HELLS
NCBI Official Synonym Symbols:	LSH; PASG; SMARCA6; Nbla10143
NCBI Protein Information:	lymphoid-specific helicase; HELLS; proliferation-associated SNF2-like protein; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6; SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6
UniProt Protein Name:	Lymphoid-specific helicase
UniProt Synonym Protein Names:	Proliferation-associated SNF2-like protein; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6
Protein Family:	Lymphoid-specific helicase
UniProt Gene Name:	HELLS
UniProt Entry Name:	HELLS_HUMAN

	Western blot analysis of extracts of various cell lines, using HELLS antibody (CAB5831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (CAB5831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (CAB5831) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HELLS antibody (CAB5831). Western blot was performed from the immunoprecipitate using HELLS antibody (CAB5831) at a dilition of 1:1000.