Metabolism Antibodies 2
Anti-HDLBP Antibody (CAB6028)
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- SKU:
- CAB6028
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-HDLBP Antibody |
| Antibody SKU: | CAB6028 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1268 of human HDLBP (NP_976221.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, HepG2, Mouse brain, NIH/3T3, Mouse heart, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1268 of human HDLBP (NP_976221.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LERV KELQ AEQE DRAL RSFK LSVT VDPK YHPK IIGR KGAV ITQI RLEH DVNI QFPD KDDG NQPQ DQIT ITGY EKNT EAAR DAIL RIVG ELEQ MVSE DVPL DHRV HARI IGAR GKAI RKIM DEFK VDIR FPQS GAPD PNCV TVTG LPEN VEEA IDHI LNLE EEYL ADVV DSEA LQVY MKPP AHEE AKAP SRGF VVRD APWT ASSS EKAP DMSS SEEF PSFG AQVA PKTL PWGP KR |
| Gene ID: | 3069 |
| Uniprot: | Q00341 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 137kDa/141kDa |
| Observed MW: | 150kDa |
| Synonyms: | HDLBP, HBP, PRO2900, VGL, vigilin |
| Background: | The protein encoded by this gene binds high density lipoprotein (HDL) and may function to regulate excess cholesterol levels in cells. The encoded protein also binds RNA and can induce heterochromatin formation. |
| UniProt Code: | Q00341 |
| NCBI GenInfo Identifier: | 218511884 |
| NCBI Gene ID: | 3069 |
| NCBI Accession: | Q00341.2 |
| UniProt Secondary Accession: | Q00341,Q53QU2, Q9UCY3, B4DTQ2, E7EM71, |
| UniProt Related Accession: | Q00341 |
| Molecular Weight: | 1268 |
| NCBI Full Name: | Vigilin |
| NCBI Synonym Full Names: | high density lipoprotein binding protein |
| NCBI Official Symbol: | HDLBP |
| NCBI Official Synonym Symbols: | HBP; VGL; PRO2900 |
| NCBI Protein Information: | vigilin; HDL-binding protein; high density lipoprotein-binding protein |
| UniProt Protein Name: | Vigilin |
| UniProt Synonym Protein Names: | High density lipoprotein-binding protein; HDL-binding protein |
| Protein Family: | Vigilin |
| UniProt Gene Name: | HDLBP |
| UniProt Entry Name: | VIGLN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HDLBP antibody (CAB6028) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat liver using HDLBP antibody (CAB6028) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver using HDLBP antibody (CAB6028) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using HDLBP antibody (CAB6028) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using HDLBP Rabbit pAb (CAB6028) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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