KO Validated Antibodies 1

Anti-HDAC8 Antibody (CAB5829)[KO Validated]

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SKU:
CAB5829
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-HDAC8 Antibody [KO Validated]
Antibody SKU:CAB5829
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEEP EEPA DSGQ SLVP VYIY SPEY VSMC DSLA KIPK RASM VHSL IEAY ALHK QMRI VKPK VASM EEMA TFHT DAYL QHLQ KVSQ EGDD DHPD SIEY GLGY DCPA TEGI FDYA AAIG GATI TAAQ CLID GMCK VAIN WSGG WHHA KKDE ASGF CYLN DAVL GILR LRRK FERI LYVD LDLH HGDG VEDA FSFT SKVM TVSL HKFS PGFF PGTG DVSD VGLG KGRY YSVN VPIQ DGIQ DEKY YQIC ESVL KEVY QAFN PKAV VLQL GADT IAGD PMCS FNMT PVGI GKCL KYIL QWQL ATLI LGGG GYNL ANTA RCWT YLTG VILG KTLS SEIP DHEF FTAY GPDY VLEI TPSC RPDR NEPH RIQQ ILNY IKGN LKHV V
Gene ID:55869
Uniprot:Q9BY41
Cellular Location:Cytoplasm, Nucleus
Calculated MW:15kDa/16kDa/17kDa/28kDa/29kDa/31kDa/41kDa
Observed MW:42kDa
Synonyms:HDAC8, CDA07, CDLS5, HD8, HDACL1, MRXS6, RPD3, WTS
Background:Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; nuclear chromosome; histone deacetylase complex; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function:NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding

Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; sister chromatid cohesion; mitotic cell cycle; chromatin modification; negative regulation of transcription from RNA polymerase II promoter

Disease: Wilson-turner X-linked Mental Retardation Syndrome

NCBI Summary:Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:Q9BY41
NCBI GenInfo Identifier:29839394
NCBI Gene ID:55869
NCBI Accession:Q9BY41.2
UniProt Secondary Accession:Q9BY41,Q86VC8, Q9NP76, Q9NYH4, A6ND12, A6ND61, A6NET3 A6NJR3, A8MQ62, B4DKN0, B4DV22,
UniProt Related Accession:Q9BY41
Molecular Weight:377
NCBI Full Name:Histone deacetylase 8
NCBI Synonym Full Names:histone deacetylase 8
NCBI Official Symbol:HDAC8  
NCBI Official Synonym Symbols:HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1  
NCBI Protein Information:histone deacetylase 8; histone deacetylase-like 1
UniProt Protein Name:Histone deacetylase 8
UniProt Gene Name:HDAC8  
UniProt Entry Name:HDAC8_HUMAN
Anti-HDAC8 Antibody [KO Validated] (CAB5829)Western blot analysis of extracts of various cell lines, using HDAC8 Antibody (CAB5829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-HDAC8 Antibody [KO Validated] (CAB5829)Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (CAB5829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-HDAC8 Antibody [KO Validated] (CAB5829)Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).
Anti-HDAC8 Antibody [KO Validated] (CAB5829)Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).
Anti-HDAC8 Antibody [KO Validated] (CAB5829)Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).
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