Epigenetics & Nuclear Signaling Antibodies 4
Anti-HDAC7 Antibody (CAB7285)
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- SKU:
- CAB7285
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HDAC7 Antibody |
| Antibody SKU: | CAB7285 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human HDAC7 (NP_056216.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3, A-549, MCF7, Jurkat, Mouse lung, Mouse heart, Mouse brain, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human HDAC7 (NP_056216.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LPPS ATAP PPPG PMQP RLEQ LKTH VQVI KRSA KPSE KPRL RQIP SAED LETD GGGP GQVV DDGL EHRE LGHG QPEA RGPA PLQQ HPQV LLWE QQRL AGRL P |
| Gene ID: | 51564 |
| Uniprot: | Q8WUI4 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 46-66kDa/99-108kDa |
| Observed MW: | 120kDa |
| Synonyms: | HDAC7, HD7, HD7A, HDAC7A |
| Background: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Positively regulates the transcriptional repressor activity of FOXP3 (PubMed:17360565). |
| NCBI Summary: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8WUI4 |
| NCBI GenInfo Identifier: | 30913097 |
| NCBI Gene ID: | 51564 |
| NCBI Accession: | Q8WUI4.2 |
| UniProt Secondary Accession: | Q8WUI4,Q6P1W9, Q6W9G7, Q7Z4K2, Q7Z5I1, Q96K01, Q9BR73 Q9H7L0, Q9NW41, B3KY08, B4DWI0, B4E0Q5, |
| UniProt Related Accession: | Q8WUI4 |
| Molecular Weight: | 66,187 Da |
| NCBI Full Name: | Histone deacetylase 7 |
| NCBI Synonym Full Names: | histone deacetylase 7 |
| NCBI Official Symbol: | HDAC7 |
| NCBI Official Synonym Symbols: | HD7; HD7A; HDAC7A |
| NCBI Protein Information: | histone deacetylase 7 |
| UniProt Protein Name: | Histone deacetylase 7 |
| UniProt Synonym Protein Names: | Histone deacetylase 7A; HD7a |
| Protein Family: | Histone deacetylase |
| UniProt Gene Name: | HDAC7 |
| UniProt Entry Name: | HDAC7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HDAC7 antibody (CAB7285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using HDAC7 antibody (CAB7285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of MCF-7 cells using HDAC7 antibody (CAB7285). Blue: DAPI for nuclear staining. |
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