Autophagy Antibodies
Anti-HDAC6 Antibody (CAB11259)
- SKU:
- CAB11259
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-HDAC6 Antibody |
| Antibody SKU: | CAB11259 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, SKOV3, HepG2, SW620, BT-474, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VMKV EDRE GPSS SKLV TKKA PQPA KPRL AERM TTRE KKVL EAGM GKVT SASF GEES TPGQ TNSE TAVV ALTQ DQPS EAAT GGAT LAQT ISEA AIGG AMLG QTTS EEAV GGAT PDQT TSEE TVGG AILD QTTS EDAV GGAT LGQT TSEE AVGG ATLA QTTS EAAM EGAT LDQT TSEE APGG TELI QTPL ASST DHQT PPTS PVQG TTPQ ISPS TLIG SLRT LELG SESQ GASE SQAP GEEN LLGE AAGG QDMA DSML MQGS RGLT DQAI F |
| Gene ID: | 10013 |
| Uniprot: | Q9UBN7 |
| Cellular Location: | Cell projection, Cytoplasm, Nucleus, Perikaryon, axon, dendrite |
| Calculated MW: | 114kDa/131kDa |
| Observed MW: | 170kDa |
| Synonyms: | HDAC6, CPBHM, HD6, JM21, PPP1R90 |
| Background: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. |
| UniProt Protein Function: | HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; EC 3.5.1.98; Nuclear receptor co-regulator; Deacetylase Chromosomal Location of Human Ortholog: Xp11.23 Cellular Component: microtubule; dendrite; histone deacetylase complex; leading edge; perikaryon; caveola; inclusion body; cytosol; nucleoplasm; dynein complex; microtubule associated complex; axon; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasm; nucleus Molecular Function:zinc ion binding; microtubule binding; histone deacetylase binding; beta-catenin binding; beta-tubulin binding; Hsp90 protein binding; misfolded protein binding; actin binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); tubulin deacetylase activity; ubiquitin protein ligase binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; polyubiquitin binding; tau protein binding; alpha-tubulin binding Biological Process: negative regulation of proteolysis; response to misfolded protein; protein polyubiquitination; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; positive regulation of signal transduction; regulation of fat cell differentiation; macroautophagy; negative regulation of microtubule depolymerization; response to toxin; organelle organization and biogenesis; misfolded or incompletely synthesized protein catabolic process; histone deacetylation; regulation of gene expression, epigenetic; response to organic substance; intracellular protein transport; protein complex disassembly; lysosome localization; protein amino acid deacetylation; negative regulation of oxidoreductase activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent; negative regulation of protein complex disassembly Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia |
| NCBI Summary: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UBN7 |
| NCBI GenInfo Identifier: | 205371758 |
| NCBI Gene ID: | 10013 |
| NCBI Accession: | Q9UBN7.2 |
| UniProt Secondary Accession: | Q9UBN7,O94975, Q6NT75, Q7L3E5, Q96CY0, |
| UniProt Related Accession: | Q9UBN7 |
| Molecular Weight: | 114,361 Da |
| NCBI Full Name: | Histone deacetylase 6 |
| NCBI Synonym Full Names: | histone deacetylase 6 |
| NCBI Official Symbol: | HDAC6 |
| NCBI Official Synonym Symbols: | HD6; JM21; CPBHM; PPP1R90 |
| NCBI Protein Information: | histone deacetylase 6; protein phosphatase 1, regulatory subunit 90 |
| UniProt Protein Name: | Histone deacetylase 6 |
| UniProt Gene Name: | HDAC6 |
| UniProt Entry Name: | HDAC6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HDAC6 antibody (CAB11259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using HDAC6 antibody (CAB11259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (CAB11259). Western blot was performed from the immunoprecipitate using HDAC6 antibody (CAB11259) at a dilition of 1:1000. |
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