Description
| Antibody Name: | Anti-HDAC2 Antibody |
| Antibody SKU: | CAB2084 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, NIH/3T3, COS-1, COS-7, HeLa, Jurkat, SH-SY5Y |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VHED SGDE DGED PDKR ISIR ASDK RIAC DEEF SDSE DEGE GGRR NVAD HKKG AKKA RIEE DKKE TEDK KTDV KEED KSKD NSGE KTDT KGTK SEQL SNP |
| Gene ID: | 3066 |
| Uniprot: | Q92769 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 51kDa/55kDa |
| Observed MW: | 60kDa |
| Synonyms: | HD2, RPD3, YAF1, HDAC2 |
| Background: | This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. |
| UniProt Protein Details: | Protein type:EC 3.5.1.98; Transcription, coactivator/corepressor; Deacetylase; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 6q21 Cellular Component: nucleoplasm; heterochromatin; transcription factor complex; protein complex; Sin3 complex; ESC/E(Z) complex; cytoplasm; nuclear chromatin; replication fork; NuRD complex; nucleus Molecular Function:deacetylase activity; nucleosomal DNA binding; protein deacetylase activity; transcription factor binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; heat shock protein binding; chromatin binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor activity Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; cardiac muscle cell development; negative regulation of cardiac muscle cell proliferation; negative regulation of gene expression, epigenetic; positive regulation of cell proliferation; dendrite development; circadian regulation of gene expression; positive regulation of oligodendrocyte differentiation; response to drug; transcription, DNA-dependent; negative regulation of transcription factor activity; hippocampus development; histone deacetylation; regulation of protein kinase B signaling cascade; response to cocaine; odontogenesis of dentine-containing teeth; chromatin remodeling; maintenance of chromatin silencing; negative regulation of MHC class II biosynthetic process; ATP-dependent chromatin remodeling; epidermal cell differentiation; positive regulation of transcription factor activity; gene expression; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; blood coagulation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis |
| NCBI Summary: | This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010] |
| UniProt Code: | Q92769 |
| NCBI GenInfo Identifier: | 68068066 |
| NCBI Gene ID: | 3066 |
| NCBI Accession: | Q92769.2 |
| UniProt Secondary Accession: | Q92769,Q5SRI8, Q5SZ86, Q8NEH4, B3KRS5, B4DL58, E1P561 |
| UniProt Related Accession: | Q92769 |
| Molecular Weight: | 488 |
| NCBI Full Name: | Histone deacetylase 2 |
| NCBI Synonym Full Names: | histone deacetylase 2 |
| NCBI Official Symbol: | HDAC2Â Â |
| NCBI Official Synonym Symbols: | HD2; RPD3; YAF1Â Â |
| NCBI Protein Information: | histone deacetylase 2; YY1-associated factor 1; transcriptional regulator homolog RPD3 |
| UniProt Protein Name: | Histone deacetylase 2 |
| UniProt Gene Name: | HDAC2Â Â |
| UniProt Entry Name: | HDAC2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HDAC2 antibody (CAB2084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (CAB2084) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (CAB2084) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (CAB2084) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using HDAC2 antibody (CAB2084). Blue: DAPI for nuclear staining. |
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