Cell Biology Antibodies 18
Anti-HDAC1 Mouse Monoclonal Antibody (CAB18304)
- SKU:
- CAB18304
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Mouse
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Antibody Name: | Anti-HDAC1 Mouse Monoclonal Antibody |
Antibody SKU: | CAB18304 |
Antibody Size: | 20uL, 50uL |
Application: | WB IHC |
Reactivity: | Human, Mouse |
Host Species: | Mouse |
Immunogen: | Recombinant protein of human HDAC1. |
Application: | WB IHC |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Reactivity: | Human, Mouse |
Positive Samples: | HeLa, 293T, BxPC-3 |
Immunogen: | Recombinant protein of human HDAC1. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Gene ID: | |
Uniprot: | |
Cellular Location: | |
Calculated MW: | |
Observed MW: | 62kDa |
Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. | |
Immunohistochemistry of paraffin-embedded Human esophageal using HDAC1 Mouse mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. | |
Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |