KO Validated Antibodies 2

Anti-HDAC1 Antibody [KO Validated] (CAB19571)

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SKU:
CAB19571
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HDAC1 Antibody [KO Validated]
Antibody SKU:CAB19571
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human HDAC1.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, MCF7, NIH/3T3, Mouse thymus, Mouse testis, Rat testis
Immunogen:A synthesized peptide derived from human HDAC1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3065
Uniprot:Q13547
Cellular Location:
Calculated MW:55kDa
Observed MW:65kDa
Anti-HDAC1 Antibody [KO Validated] (CAB19571)Western blot analysis of extracts of various cell lines, using HDAC1 antibody (CAB19571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-HDAC1 Antibody [KO Validated] (CAB19571)Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (CAB19571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-HDAC1 Antibody [KO Validated] (CAB19571)Immunohistochemistry of paraffin-embedded Rat testis using HDAC1 antibody (CAB19571) at dilution of 1:100 (40x lens).
Anti-HDAC1 Antibody [KO Validated] (CAB19571)Immunohistochemistry of paraffin-embedded Mouse brain using HDAC1 antibody (CAB19571) at dilution of 1:100 (40x lens).
Anti-HDAC1 Antibody [KO Validated] (CAB19571)Immunofluorescence analysis of C6 cells using HDAC1 antibody (CAB19571) at dilution of 1:100. Blue: DAPI for nuclear staining.
Synonyms:GON-10, HD1, RPD3, RPD3L1, HDAC1
Background:Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
UniProt Protein Function:HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: chromatin; cytoplasm; cytosol; histone deacetylase complex; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; protein complex; Sin3 complex

Molecular Function:deacetylase activity; enzyme binding; histone deacetylase activity; histone deacetylase binding; NF-kappaB binding; nucleosomal DNA binding; protein binding; protein deacetylase activity; protein N-terminus binding; transcription activator binding; transcription factor activity; transcription factor binding

Biological Process: ATP-dependent chromatin remodeling; blood coagulation; chromatin modification; chromatin remodeling; circadian regulation of gene expression; embryonic digit morphogenesis; epidermal cell differentiation; histone deacetylation; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid deacetylation

NCBI Summary:Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
UniProt Code:Q13547
NCBI GenInfo Identifier:2498443
NCBI Gene ID:3065
NCBI Accession:Q13547.1
UniProt Secondary Accession:Q13547,Q92534,
UniProt Related Accession:Q13547
Molecular Weight:55,103 Da
NCBI Full Name:Histone deacetylase 1
NCBI Synonym Full Names:histone deacetylase 1
NCBI Official Symbol:HDAC1  
NCBI Official Synonym Symbols:HD1; RPD3; GON-10; RPD3L1  
NCBI Protein Information:histone deacetylase 1
UniProt Protein Name:Histone deacetylase 1
UniProt Gene Name:HDAC1  
UniProt Entry Name:HDAC1_HUMAN
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