Antibody Name:	Anti-HDAC1 Antibody [KO Validated]
Antibody SKU:	CAB0238
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, HepG2, MCF7, Mouse thymus, Mouse testis, Rat thymus, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SGDE DEDD PDKR ISIC SSDK RIAC EEEF SDSE EEGE GGRK NSSN FKKA KRVK TEDE KEKD PEEK KEVT EEEK TKEE KPEA KGVK EEVK LA
Gene ID:	3065
Uniprot:	Q13547
Cellular Location:	Nucleus
Calculated MW:	55kDa
Observed MW:	62kDa

Synonyms:

GON-10, HD1, RPD3, RPD3L1, HDAC1

Background:

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

UniProt Protein Function:	HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; EC 3.5.1.98; Hydrolase Chromosomal Location of Human Ortholog: 1p34 Cellular Component: chromatin; cytoplasm; cytosol; histone deacetylase complex; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; protein complex; Sin3 complex Molecular Function:deacetylase activity; enzyme binding; histone deacetylase activity; histone deacetylase binding; NF-kappaB binding; nucleosomal DNA binding; protein binding; protein deacetylase activity; protein N-terminus binding; transcription activator binding; transcription factor activity; transcription factor binding Biological Process: ATP-dependent chromatin remodeling; blood coagulation; chromatin modification; chromatin remodeling; circadian regulation of gene expression; embryonic digit morphogenesis; epidermal cell differentiation; histone deacetylation; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid deacetylation
NCBI Summary:	Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13547
NCBI GenInfo Identifier:	2498443
NCBI Gene ID:	3065
NCBI Accession:	Q13547.1
UniProt Secondary Accession:	Q13547,Q92534,
UniProt Related Accession:	Q13547
Molecular Weight:	55,103 Da
NCBI Full Name:	Histone deacetylase 1
NCBI Synonym Full Names:	histone deacetylase 1
NCBI Official Symbol:	HDAC1
NCBI Official Synonym Symbols:	HD1; RPD3; GON-10; RPD3L1
NCBI Protein Information:	histone deacetylase 1
UniProt Protein Name:	Histone deacetylase 1
UniProt Gene Name:	HDAC1
UniProt Entry Name:	HDAC1_HUMAN

	Western blot analysis of extracts of various cell lines, using HDAC1 antibody (CAB0238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (CAB0238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] HDAC1 Rabbit pAb (CAB0238) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using [KO Validated] HDAC1 Rabbit pAb (CAB0238) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using [KO Validated] HDAC1 Rabbit pAb (CAB0238) at dilution of 1:50 (40x lens).

Antibodies	ELISA Kits
Anti-HDAC1 Antibody [KO Validated] (CAB19571)	Human Histone deacetylase 1 (HDAC1) ELISA Kit
HDAC1 Antibody (PACO00899)	HDAC1 Colorimetric Cell-Based ELISA Kit
HDAC1 Antibody (PACO02170)	HDAC1 (Phospho-Ser421) Colorimetric Cell-Based ELISA Kit
Phospho-HDAC1 (S421) Antibody (PACO04040)
HDAC1 Antibody (PACO06546)