Cell Biology Antibodies 8

Anti-HCRTR2 Antibody (CAB3057)

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SKU:
CAB3057
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HCRTR2 Antibody
Antibody SKU:CAB3057
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 380-444 of human HCRTR2 (NP_001517.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, 293T, Mouse brain, Mouse heart, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 380-444 of human HCRTR2 (NP_001517.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SCCC LGVH HRQE DRLT RGRT STES RKSL TTQI SNFD NISK LSEQ VVLT SIST LPAA NGAG PLQN W
Gene ID:3062
Uniprot:O43614
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:50kDa
Observed MW:50kDa
Synonyms:HCRTR2, OX2R
Background:The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A.
UniProt Protein Function:HCRTR2: Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:

Protein type:GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function:neuropeptide receptor activity; orexin receptor activity; peptide hormone binding; peptide binding

Biological Process: synaptic transmission; neuropeptide signaling pathway; circadian sleep/wake cycle process; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); cellular response to hormone stimulus; regulation of circadian sleep/wake cycle, sleep

NCBI Summary:The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A. [provided by RefSeq, Jan 2009]
UniProt Code:O43614
NCBI GenInfo Identifier:206729885
NCBI Gene ID:3062
NCBI Accession:O43614.2
UniProt Secondary Accession:O43614,P58308,
UniProt Related Accession:O43614
Molecular Weight:444
NCBI Full Name:Orexin receptor type 2
NCBI Synonym Full Names:hypocretin (orexin) receptor 2
NCBI Official Symbol:HCRTR2  
NCBI Official Synonym Symbols:OX2R  
NCBI Protein Information:orexin receptor type 2; ox2-R; ox-2-R; orexin type-2 receptor; hypocretin receptor type 2
UniProt Protein Name:Orexin receptor type 2
UniProt Synonym Protein Names:Hypocretin receptor type 2
UniProt Gene Name:HCRTR2  
UniProt Entry Name:OX2R_HUMAN
Anti-HCRTR2 Antibody (CAB3057)Western blot analysis of extracts of various cell lines, using HCRTR2 antibody (CAB3057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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