Cell Biology Antibodies 5

Anti-HCRTR1 Antibody (CAB14740)

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SKU:
CAB14740
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HCRTR1 Antibody
Antibody SKU:CAB14740
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HPLL FKST ARRA RGSI LGIW AVSL AIMV PQAA VMEC SSVL PELA NRTR LFSV CDER WADD LYPK IYHS CFFI VTYL APLG LMAM AYFQ IFRK LWGR QIPG T
Gene ID:3061
Uniprot:O43613
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:47kDa
Observed MW:47kDa
Synonyms:HCRTR1, OX1R
Background:The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B.
UniProt Protein Function:HCRTR1: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:

Protein type:Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function:G-protein coupled receptor activity; neuropeptide receptor activity; orexin receptor activity; peptide binding

Biological Process: cellular response to hormone stimulus; regulation of circadian sleep/wake cycle, sleep; synaptic transmission

NCBI Summary:The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009]
UniProt Code:O43613
NCBI GenInfo Identifier:205804177
NCBI Gene ID:3061
NCBI Accession:O43613.2
UniProt Secondary Accession:O43613,Q9HBV6, A8K3A6,
UniProt Related Accession:O43613
Molecular Weight:47,536 Da
NCBI Full Name:Orexin receptor type 1
NCBI Synonym Full Names:hypocretin receptor 1
NCBI Official Symbol:HCRTR1  
NCBI Official Synonym Symbols:OX1R  
NCBI Protein Information:orexin receptor type 1
UniProt Protein Name:Orexin receptor type 1
UniProt Synonym Protein Names:Hypocretin receptor type 1
Protein Family:Orexin receptor
UniProt Gene Name:HCRTR1  
UniProt Entry Name:OX1R_HUMAN
Anti-HCRTR1 Antibody (CAB14740)Western blot analysis of extracts of various cell lines, using HCRTR1 antibody (CAB14740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-HCRTR1 Antibody (CAB14740)Immunofluorescence analysis of mouse brain using HCRTR1 Rabbit pAb (CAB14740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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