Cell Death Antibodies 1

Anti-HCAR2 Antibody (CAB15611)

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SKU:
CAB15611
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-HCAR2 Antibody
Antibody SKU:CAB15611
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HCAR2 (NP_808219.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, Rat heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HCAR2 (NP_808219.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VRIR IFWL LHTS GTQN CEVY RSVD LAFF ITLS FTYM NSML DPVV YYFS SPSF PNFF STLI NRCL QRKM TGEP DNNR STSV ELTG DPNK TRGA PEAL MANS G
Gene ID:338442
Uniprot:Q8TDS4
Cellular Location:
Calculated MW:42kDa
Observed MW:42kDa
Synonyms:HCAR2, GPR109A, HCA2, HM74a, HM74b, NIACR1, PUMAG, Puma-g
Background:
UniProt Protein Function:GPR109A: Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5- methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:

Protein type:Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell junction; plasma membrane

Biological Process: negative regulation of lipid catabolic process; neutrophil apoptosis; positive regulation of neutrophil apoptosis

UniProt Code:Q8TDS4
NCBI GenInfo Identifier:74762622
NCBI Gene ID:338442
NCBI Accession:Q8TDS4.1
UniProt Secondary Accession:Q8TDS4,A0PJL5, A7LGG3,
UniProt Related Accession:Q8TDS4
Molecular Weight:41,850 Da
NCBI Full Name:Hydroxycarboxylic acid receptor 2
NCBI Synonym Full Names:hydroxycarboxylic acid receptor 2
NCBI Official Symbol:HCAR2  
NCBI Official Synonym Symbols:HCA2; HM74a; HM74b; PUMAG; NIACR1; Puma-g; GPR109A  
NCBI Protein Information:hydroxycarboxylic acid receptor 2
UniProt Protein Name:Hydroxycarboxylic acid receptor 2
UniProt Synonym Protein Names:G-protein coupled receptor 109A; G-protein coupled receptor HM74A; Niacin receptor 1; Nicotinic acid receptor
Protein Family:Hydroxycarboxylic acid receptor
UniProt Gene Name:HCAR2  
UniProt Entry Name:HCAR2_HUMAN
Anti-HCAR2 Antibody (CAB15611)Western blot analysis of extracts of various cell lines, using HCAR2 antibody (CAB15611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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