KO Validated Antibodies 1

Anti-HB EGF[KO Validated] Antibody (CAB11657)

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SKU:
CAB11657
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HB EGF[KO Validated] Antibody
Antibody SKU:CAB11657
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human HB EGF
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse lung, Mouse spleen, Rat spleen
Immunogen:A synthesized peptide derived from human HB EGF
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1839
Uniprot:Q99075
Cellular Location:
Calculated MW:23kDa
Observed MW:25KDa
Anti-HB EGF[KO Validated] AntibodyWestern blot analysis of extracts from normal (control) and HB EGF knockout (KO) 293T cells, using HB EGF Rabbit mAb (CAB11657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-HB EGF[KO Validated] AntibodyWestern blot analysis of extracts of various cell lines, using HB EGF Rabbit mAb (CAB11657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:DTR, DTS, DTSF, HEGFL
Background:
UniProt Protein Function:HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function:heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; positive regulation of cell migration; wound healing, spreading of epidermal cells

UniProt Code:Q99075
NCBI GenInfo Identifier:544477
NCBI Gene ID:1839
NCBI Accession:Q99075.1
UniProt Secondary Accession:Q99075,B2R821,
UniProt Related Accession:Q99075
Molecular Weight:Calculated MW: 23kDaObserved MW: 23kDa
NCBI Full Name:Proheparin-binding EGF-like growth factor
NCBI Synonym Full Names:heparin-binding EGF-like growth factor
NCBI Official Symbol:HBEGF  
NCBI Official Synonym Symbols:DTR; DTS; DTSF; HEGFL  
NCBI Protein Information:proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
UniProt Protein Name:Proheparin-binding EGF-like growth factor
UniProt Synonym Protein Names:Diphtheria toxin receptor; DT-R
Protein Family:Proheparin-binding EGF-like growth factor
UniProt Gene Name:HBEGF  
UniProt Entry Name:HBEGF_HUMAN
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