Antibody Name:	Anti-HARS2 Antibody
Antibody SKU:	CAB7785
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 217-506 of human HARS2 (NP_036340.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 217-506 of human HARS2 (NP_036340.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IVDG MFAV CGVP ESKF RAIC SSID KLDK MAWK DVRH EMVV KKGL APEV ADRI GDYV QCHG GVSL VEQM FQDP RLSQ NKQA LEGL GDLK LLFE YLTL FGIA DKIS FDLS LARG LDYY TGVI YEAV LLQT PTQA GEEP LNVG SVAA GGRY DGLV GMFD PKGH KVPC VGLS IGVE RIFY IVEQ RMKT KGEK VRTT ETQV FVAT PQKN FLQE RLKL IAEL WDSG IKAE MLYK NNPK LLTQ LHYC ESTG IPLV VIIG EQEL KEGV IKIR SVAS REEV AIKR ENFV AEIQ KRLS ES
Gene ID:	23438
Uniprot:	P49590
Cellular Location:	Mitochondrion matrix
Calculated MW:	54kDa/56kDa
Observed MW:	50kDa

Synonyms:

HARS2, HARSL, HARSR, HO3, PRLTS2

Background:

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.

UniProt Protein Function:	HARS2: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes share a bidirectional promoter. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Aminoacyl-tRNA synthetase; EC 6.1.1.21; Ligase; Mitochondrial; Translation; Translation regulation Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:histidine-tRNA ligase activity; protein binding; protein homodimerization activity; RNA binding Biological Process: histidyl-tRNA aminoacylation; mitochondrial translation; tRNA aminoacylation for protein translation Disease: Perrault Syndrome 2
NCBI Summary:	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
UniProt Code:	P49590
NCBI GenInfo Identifier:	523567531
NCBI Gene ID:	23438
NCBI Accession:	NP_001265660.1
UniProt Secondary Accession:	P49590,B4DDY8,
UniProt Related Accession:	P49590
Molecular Weight:	69.3kD
NCBI Full Name:	probable histidine--tRNA ligase, mitochondrial isoform 2
NCBI Synonym Full Names:	histidyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol:	HARS2
NCBI Official Synonym Symbols:	HO3; HARSL; HARSR; PRLTS2
NCBI Protein Information:	probable histidine--tRNA ligase, mitochondrial
UniProt Protein Name:	Probable histidine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names:	Histidine--tRNA ligase-like; Histidyl-tRNA synthetase; HisRS
Protein Family:	Probable histidine--tRNA ligase
UniProt Gene Name:	HARS2

	Western blot analysis of extracts of various cell lines, using HARS2 antibody (CAB7785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using HARS2 antibody (CAB7785) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using HARS2 antibody (CAB7785) at dilution of 1:100 (40x lens).