Metabolism Antibodies 2

Anti-HARS Antibody (CAB7899)

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SKU:
CAB7899
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-HARS Antibody
Antibody SKU:CAB7899
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HARS (NP_002100.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:MCF7, HeLa, U-251MG, NCI-H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HARS (NP_002100.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAER AALE ELVK LQGE RVRG LKQQ KASA ELIE EEVA KLLK LKAQ LGPD ESKQ KFVL KTPK GTRD YSPR QMAV REKV FDVI IRCF KRHG AEVI DTPV FELK ETLM GKYG EDSK LIYD LKDQ GGEL LSLR YDLT VPFA RYLA MNKL TNIK RYHI AKVY RRDN PAMT RGRY REFY QCDF DIAG
Gene ID:3035
Uniprot:P12081
Cellular Location:Cytoplasm
Calculated MW:50kDa/52kDa/54kDa/57kDa
Observed MW:57kDa
Synonyms:HARS, CMT2W, HRS, USH3B
Background:Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:HARS: Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family.
UniProt Protein Details:

Protein type:EC 6.1.1.21; Translation; Ligase

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytoplasm; cytosol; mitochondrion

Molecular Function:histidine-tRNA ligase activity

Biological Process: histidyl-tRNA aminoacylation; mitochondrial translation; tRNA aminoacylation for protein translation

Disease: Charcot-marie-tooth Disease, Axonal, Type 2w; Usher Syndrome, Type Iiib

NCBI Summary:Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:P12081
NCBI GenInfo Identifier:135123
NCBI Gene ID:3035
NCBI Accession:P12081.2
UniProt Secondary Accession:P12081,B4DHQ1, B4DY73, D6REN6, J3KNE5,
UniProt Related Accession:P12081
Molecular Weight:54,847 Da
NCBI Full Name:Histidine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:histidyl-tRNA synthetase
NCBI Official Symbol:HARS  
NCBI Official Synonym Symbols:HRS; CMT2W; USH3B  
NCBI Protein Information:histidine--tRNA ligase, cytoplasmic
UniProt Protein Name:Histidine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Histidyl-tRNA synthetase; HisRS
Protein Family:Histidine--tRNA ligase
UniProt Gene Name:HARS  
UniProt Entry Name:SYHC_HUMAN
Anti-HARS Antibody (CAB7899)Western blot analysis of extracts of various cell lines, using HARS antibody (CAB7899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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