Cell Biology Antibodies 10
Anti-HAPLN1 Antibody (CAB6616)
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- SKU:
- CAB6616
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HAPLN1 Antibody |
| Antibody SKU: | CAB6616 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 165-354 of human HAPLN1 (NP_001875.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, A375, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 165-354 of human HAPLN1 (NP_001875.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PRLG RYNL NFHE AQQA CLDQ DAVI ASFD QLYD AWRG GLDW CNAG WLSD GSVQ YPIT KPRE PCGG QNTV PGVR NYGF WDKD KSRY DVFC FTSN FNGR FYYL IHPT KLTY DEAV QACL NDGA QIAK VGQI FAAW KILG YDRC DAGW LADG SVRY PISR PRRR CSPT EAAV RFVG FPDK KHKL YGVY CFRA YN |
| Gene ID: | 1404 |
| Uniprot: | P10915 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 40kDa |
| Observed MW: | 50kDa |
| Synonyms: | HAPLN1, CRT1, CRTL1 |
| Background: |
| UniProt Protein Function: | HAPLN1: Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Belongs to the HAPLN family. |
| UniProt Protein Details: | Protein type:Extracellular matrix Chromosomal Location of Human Ortholog: 5q14.3 Cellular Component: extracellular matrix; extracellular region; proteinaceous extracellular matrix Molecular Function:extracellular matrix structural constituent; hyaluronic acid binding Biological Process: central nervous system development; extracellular matrix organization and biogenesis; skeletal development |
| UniProt Code: | P10915 |
| NCBI GenInfo Identifier: | 130310 |
| NCBI Gene ID: | 1404 |
| NCBI Accession: | P10915.2 |
| UniProt Secondary Accession: | P10915,B2R9A9, |
| UniProt Related Accession: | P10915 |
| Molecular Weight: | 40kDa |
| NCBI Full Name: | Hyaluronan and proteoglycan link protein 1 |
| NCBI Synonym Full Names: | hyaluronan and proteoglycan link protein 1 |
| NCBI Official Symbol: | HAPLN1 |
| NCBI Official Synonym Symbols: | CRT1; CRTL1 |
| NCBI Protein Information: | hyaluronan and proteoglycan link protein 1 |
| UniProt Protein Name: | Hyaluronan and proteoglycan link protein 1 |
| UniProt Synonym Protein Names: | Cartilage-linking protein 1; Cartilage-link protein; Proteoglycan link protein |
| Protein Family: | Hyaluronan and proteoglycan link protein |
| UniProt Gene Name: | HAPLN1 |
 | Western blot analysis of extracts of various cell lines, using HAPLN1 antibody (CAB6616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using HAPLN1 antibody (CAB6616) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using HAPLN1 antibody (CAB6616) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using HAPLN1 antibody (CAB6616). Blue: DAPI for nuclear staining. |
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