Antibody Name:	Anti-HAO1 Antibody
Antibody SKU:	CAB6470
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human HAO1 (NP_060015.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human HAO1 (NP_060015.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLPR LICI NDYE QHAK SVLP KSIY DYYR SGAN DEET LADN IAAF SRWK LYPR MLRN VAET DLST SVLG QRVS MPIC VGAT AMQR MAHV DGEL ATVR ACQS LGTG MMLS SWAT SSIE EVAE AGPE ALRW LQLY IYKD REVT KKLV RQAE KMGY KAIF VTVD TPYL GNRL DDVR NRFK LPPQ LRMK NFET STLS FSPE ENFG DDSG LAAY VAKA IDPS ISWE DIKW LRRL TSLP IVAK GILR GDDA REAV KHGL NGIL VSNH GARQ LDGV PATI DVLP EIVE AVEG KVEV FLDG GVRK GTDV LKAL ALGA KAVF VGRP IVWG LAFQ GEKG VQDV LEIL KEEF RLAM ALSG CQNV KVID KTLV RKNP LAVS KI
Gene ID:	54363
Uniprot:	Q9UJM8
Cellular Location:	Peroxisome
Calculated MW:	40kDa
Observed MW:	41kDa

Synonyms:

HAO1, GOX, GOX1, HAOX1

Background:

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.

UniProt Protein Function:	HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.1.3.15; Carbohydrate Metabolism - glyoxylate and dicarboxylate Chromosomal Location of Human Ortholog: 20p12 Cellular Component: peroxisomal matrix; peroxisome Molecular Function:glycolate oxidase activity; FMN binding; glyoxylate oxidase activity; (S)-2-hydroxy-acid oxidase activity; receptor binding Biological Process: glyoxylate metabolic process; response to oxidative stress; glycolate catabolic process; fatty acid alpha-oxidation
NCBI Summary:	This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UJM8
NCBI GenInfo Identifier:	13124294
NCBI Gene ID:	54363
NCBI Accession:	Q9UJM8.1
UniProt Secondary Accession:	Q9UJM8,Q14CQ0, Q9UPZ0, Q9Y3I7,
UniProt Related Accession:	Q9UJM8
Molecular Weight:	370
NCBI Full Name:	Hydroxyacid oxidase 1
NCBI Synonym Full Names:	hydroxyacid oxidase (glycolate oxidase) 1
NCBI Official Symbol:	HAO1
NCBI Official Synonym Symbols:	GOX; GOX1; HAOX1
NCBI Protein Information:	hydroxyacid oxidase 1; glycolate oxidase; (S)-2-hydroxy-acid oxidase
UniProt Protein Name:	Hydroxyacid oxidase 1
UniProt Synonym Protein Names:	Glycolate oxidase; GOX
Protein Family:	Hydroxyacid oxidase
UniProt Gene Name:	HAO1
UniProt Entry Name:	HAOX1_HUMAN

	Western blot analysis of extracts of various cell lines, using HAO1 antibody (CAB6470) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using HAO1 antibody (CAB6470) at dilution of 1:200 (40x lens).