Name: Anti-HAND1 Antibody (CAB9855)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB9855
Availability: OutOfStock

Antibody Name:	Anti-HAND1 Antibody
Antibody SKU:	CAB9855
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-215 of human HAND1 (NP_004812.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	SH-SY5Y, A-549, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-215 of human HAND1 (NP_004812.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PHPA HPML HEPF LFGP ASRC HQER PYFQ SWLL SPAD AAPD FPAG GPPP AAAA AATA YGPD ARPG QSPG RLEA LGGR LGRR KGSG PKKE RRRT ESIN SAFA ELRE CIPN VPAD TKLS KIKT LRLA TSYI AYLM DVLA KDAQ SGDP EAFK AELK KADG GRES KRKR ELQQ HEGF PPAL GPVE KRIK GRTG WPQQ VWAL ELNQ
Gene ID:	9421
Uniprot:	O96004
Cellular Location:	Nucleus, nucleolus, nucleoplasm
Calculated MW:	23kDa
Observed MW:	30kDa

Synonyms:

HAND1, Hxt, Thing1, bHLHa27, eHand

Background:

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.

UniProt Protein Function:	HAND1: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box).
UniProt Protein Details:	Protein type:Transcription factor; Nucleolus; Cell development/differentiation; DNA-binding Chromosomal Location of Human Ortholog: 5q33 Cellular Component: cytoplasm; nucleus Molecular Function:bHLH transcription factor binding; identical protein binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor binding Biological Process: angiogenesis; blastocyst development; embryonic heart tube development; heart development; heart looping; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; trophectodermal cell differentiation; ventricular cardiac muscle morphogenesis
NCBI Summary:	The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]
UniProt Code:	O96004
NCBI GenInfo Identifier:	10720015
NCBI Gene ID:	9421
NCBI Accession:	O96004.1
UniProt Related Accession:	O96004
Molecular Weight:	23,627 Da
NCBI Full Name:	Heart- and neural crest derivatives-expressed protein 1
NCBI Synonym Full Names:	heart and neural crest derivatives expressed 1
NCBI Official Symbol:	HAND1
NCBI Official Synonym Symbols:	Hxt; eHand; Thing1; bHLHa27
NCBI Protein Information:	heart- and neural crest derivatives-expressed protein 1
UniProt Protein Name:	Heart- and neural crest derivatives-expressed protein 1
UniProt Synonym Protein Names:	Class A basic helix-loop-helix protein 27; bHLHa27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; eHAND
Protein Family:	Heart- and neural crest derivatives-expressed protein
UniProt Gene Name:	HAND1
UniProt Entry Name:	HAND1_HUMAN

Anti-HAND1 Polyclonal Antibody (CAB9855)

Western blot analysis of extracts of various cell lines, using HAND1 antibody (CAB9855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

Phospho-HAND1 (S98) Antibody (PACO05761)

HAND1 Antibody (PACO05762)

HAND1 Antibody (PACO05763)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)