KO Validated Antibodies 2

Anti-HAGH Antibody (CAB19915)[KO Validated]

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SKU:
CAB19915
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HAGH Antibody [KO Validated]
Antibody SKU:CAB19915
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human HAGH.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human HAGH.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3029
Uniprot:Q16775
Cellular Location:Cytoplasm, Mitochondrion matrix
Calculated MW:25kDa/28kDa/33kDa
Observed MW:30KDa
Anti-HAGH Antibody [KO Validated] (CAB19915)Western blot analysis of extracts from normal (control) and HAGH knockout (KO) HeLa cells, using HAGH antibody (CAB19915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:HAGH, GLO2, GLX2, GLXII, HAGH1
Background:The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:HAGH: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid. Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; EC 3.1.2.6; Carbohydrate Metabolism - pyruvate; Hydrolase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial matrix; cytoplasm

Molecular Function:hydroxyacylglutathione hydrolase activity; zinc ion binding

Biological Process: glutathione biosynthetic process

Disease: Hydroxyacyl Glutathione Hydrolase Deficiency

NCBI Summary:The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
UniProt Code:Q16775
NCBI GenInfo Identifier:257051015
NCBI Gene ID:3029
NCBI Accession:Q16775.2
UniProt Secondary Accession:Q16775,A8K290, B4DP33, B4DRA7, E7EN93,
UniProt Related Accession:Q16775
Molecular Weight:308
NCBI Full Name:Hydroxyacylglutathione hydrolase, mitochondrial
NCBI Synonym Full Names:hydroxyacylglutathione hydrolase
NCBI Official Symbol:HAGH  
NCBI Official Synonym Symbols:GLO2; GLX2; GLXII; HAGH1  
NCBI Protein Information:hydroxyacylglutathione hydrolase, mitochondrial; glx II; glyoxalase II; hydroxyacylglutathione hydroxylase
UniProt Protein Name:Hydroxyacylglutathione hydrolase, mitochondrial
UniProt Synonym Protein Names:Glyoxalase II; Glx II
UniProt Gene Name:HAGH  
UniProt Entry Name:GLO2_HUMAN
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