Cell Cycle Antibodies 2
Anti-HACE1 Antibody (CAB9593)
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- SKU:
- CAB9593
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-HACE1 Antibody |
| Antibody SKU: | CAB9593 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HACE1 (NP_065822.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HeLa, Mouse brain, Mouse lung, Mouse spleen, Mouse testis |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HACE1 (NP_065822.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MERA MEQL NRLT RSLR RART VELP EDNE TAVY TLMP MVMA DQHR SVSE LLSN SKFD VNYA FGRV KRSL LHIA ANCG SVEC LVLL LKKG ANPN YQDI SGCT |
| Gene ID: | 57531 |
| Uniprot: | Q8IYU2 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Golgi stack membrane |
| Calculated MW: | 35kDa/63kDa/77kDa/102kDa |
| Observed MW: | 102kDa |
| Synonyms: | HACE1, SPPRS |
| Background: | This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. |
| UniProt Protein Function: | HAS1: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Also able to catalyze the synthesis of chito- oligosaccharide depending on the substrate. Belongs to the NodC/HAS family. |
| UniProt Protein Details: | Protein type:Cell adhesion; EC 2.4.1.212; Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, multi-pass; Transferase Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: cytoplasm; integral to plasma membrane; plasma membrane Molecular Function:hyaluronan synthase activity Biological Process: carbohydrate metabolic process; cell adhesion; extracellular polysaccharide biosynthetic process; glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; hyaluronan biosynthetic process; hyaluronan metabolic process |
| NCBI Summary: | This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q8IYU2 |
| NCBI GenInfo Identifier: | 116875852 |
| NCBI Gene ID: | 57531 |
| NCBI Accession: | NP_065822 |
| UniProt Related Accession: | Q8IYU2 |
| Molecular Weight: | 76kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase HACE1 isoform a |
| NCBI Synonym Full Names: | HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 |
| NCBI Official Symbol: | HACE1 |
| NCBI Official Synonym Symbols: | SPPRS |
| NCBI Protein Information: | E3 ubiquitin-protein ligase HACE1 |
| UniProt Protein Name: | Hyaluronan synthase 1 |
| UniProt Synonym Protein Names: | Hyaluronate synthase 1; Hyaluronic acid synthase 1; HA synthase 1; HuHAS1 |
| Protein Family: | Hyaluronan synthase |
| UniProt Gene Name: | HAS1 |
| UniProt Entry Name: | HYAS1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HACE1 antibody (CAB9593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human placenta using HACE1 antibody (CAB9593) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using HACE1 antibody (CAB9593) at dilution of 1:100 (40x lens). |
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