Antibody Name:	Anti-HABP2 Antibody
Antibody SKU:	CAB6919
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-313 of human HABP2 (NP_004123.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	LO2, Mouse serum

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-313 of human HABP2 (NP_004123.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FSLM SLLE SLDP DWTP DQYD YSYE DYNQ EENT SSTL THAE NPDW YYTE DQAD PCQP NPCE HGGD CLVH GSTF TCSC LAPF SGNK CQKV QNTC KDNP CGRG QCLI TQSP PYYR CVCK HPYT GPSC SQVV PVCR PNPC QNGA TCSR HKRR SKFT CACP DQFK GKFC EIGS DDCY VGDG YSYR GKMN RTVN QHAC LYWN SHLL LQEN YNMF MEDA ETHG IGEH NFCR NPDA DEKP WCFI KVTN DKVK WEYC DVSA CSAQ DVAY PEES PTEP STKL PGFD SCGK TEIA ERKI KR
Gene ID:	3026
Uniprot:	Q14520
Cellular Location:	Secreted
Calculated MW:	59kDa/62kDa
Observed MW:	27kDa

Synonyms:

HABP2, FSAP, HABP, HGFAL, NMTC5, PHBP

Background:

This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by hepatocytes and proteolytically processed to generate heavy and light chains that form the mature heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This extracellular protease binds hyaluronic acid and may play a role in the coagulation and fibrinolysis systems. Mutations in this gene are associated with nonmedullary thyroid cancer and susceptibility to venous thromboembolism. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

UniProt Protein Function:	HABP2: Cleaves the alpha-chain at multiple sites and the beta- chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro- urokinase) to the active two chain form. Activates coagulation factor VII. Belongs to the peptidase S1 family.
UniProt Protein Details:	Protein type:Protease; Secreted; Secreted, signal peptide; Cell adhesion; EC 3.4.21.-; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10q25.3 Cellular Component: extracellular space; extracellular region Molecular Function:glycosaminoglycan binding; serine-type endopeptidase activity Biological Process: cell adhesion; proteolysis Disease: Thrombophilia Due To Thrombin Defect; Thyroid Cancer, Nonmedullary, 5
NCBI Summary:	The protein encoded by this gene is an extracellular serine protease that binds hyaluronic acid and is involved in cell adhesion. The encoded protein is synthesized as a single chain, but then undergoes an autoproteolytic event to form the functional heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This protease is known to cleave urinary plasminogen activator, coagulation factor VII, and the alpha and beta chains of fibrinogen, but not prothrombin, plasminogen, or the gamma chain of fibrinogen. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
UniProt Code:	Q14520
NCBI GenInfo Identifier:	73919921
NCBI Gene ID:	3026
NCBI Accession:	Q14520.1
UniProt Secondary Accession:	Q14520,O00663, A8K467, B7Z8U5, F5H5M6,
UniProt Related Accession:	Q14520
Molecular Weight:	560
NCBI Full Name:	Hyaluronan-binding protein 2
NCBI Synonym Full Names:	hyaluronan binding protein 2
NCBI Official Symbol:	HABP2
NCBI Official Synonym Symbols:	FSAP; HABP; PHBP; HGFAL
NCBI Protein Information:	hyaluronan-binding protein 2; factor VII activating protein; factor VII-activating protease; factor seven-activating protease; hyaluronic acid binding protein 2; plasma hyaluronan binding protein; plasma hyaluronan-binding protein; hepatocyte growth factor activator-like protein
UniProt Protein Name:	Hyaluronan-binding protein 2
UniProt Synonym Protein Names:	Factor VII-activating protease; Factor seven-activating protease; FSAP; Hepatocyte growth factor activator-like protein; Plasma hyaluronan-binding proteinCleaved into the following 4 chains:Hyaluronan-binding protein 2 50 kDa heavy chain; Hyaluronan-binding protein 2 50 kDa heavy chain alternate form; Hyaluronan-binding protein 2 27 kDa light chain; Hyaluronan-binding protein 2 27 kDa light chain alternate form
Protein Family:	Hyaluronan-binding protein
UniProt Gene Name:	HABP2
UniProt Entry Name:	HABP2_HUMAN

	Western blot analysis of extracts of various cell lines, using HABP2 antibody (CAB6919) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of MCF7 cells using HABP2 antibody (CAB6919). Blue: DAPI for nuclear staining.