Name: Anti-GZMH Antibody (CAB6154)
Brand: Cell Biology Antibodies 10
SKU: CAB6154
Availability: OutOfStock

Antibody Name:	Anti-GZMH Antibody
Antibody SKU:	CAB6154
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-246 of human GZMH (NP_219491.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Mouse spleen, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-246 of human GZMH (NP_219491.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EIIG GHEA KPHS RPYM AFVQ FLQE KSRK RCGG ILVR KDFV LTAA HCQG SSIN VTLG AHNI KEQE RTQQ FIPV KRPI PHPA YNPK NFSN DIML LQLE RKAK WTTA VRPL RLPS SKAQ VKPG QLCS VAGW GYVS MSTL ATTL QEVL LTVQ KDCQ CERL FHGN YSRA TEIC VGDP KKTQ TGFK GDSG GPLV CKDV AQGI LSYG NKKG TPPG VYIK VSHF LPWI KRTM KRL
Gene ID:	2999
Uniprot:	P20718
Cellular Location:	Cytoplasmic granule
Calculated MW:	12kDa/17kDa/27kDa
Observed MW:	37kDa

Synonyms:

GZMH, CCP-X, CGL-2, CSP-C, CTLA1, CTSGL2

Background:

This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14.

UniProt Protein Function:	GZMF: Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Belongs to the peptidase S1 family. Granzyme subfamily.
UniProt Protein Details:	Protein type:Protease; EC 3.4.21.- Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: cytoplasm; intracellular membrane-bound organelle; membrane Molecular Function:serine-type endopeptidase activity Biological Process: immune response; protein processing
NCBI Summary:	This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015]
UniProt Code:	P20718
NCBI GenInfo Identifier:	121590
NCBI Gene ID:	2999
NCBI Accession:	P20718.1
UniProt Secondary Accession:	P20718,Q6XGZ0, Q6XGZ1, G3V2C5,
UniProt Related Accession:	P20718
Molecular Weight:	12,609 Da
NCBI Full Name:	Granzyme H
NCBI Synonym Full Names:	granzyme H
NCBI Official Symbol:	GZMH
NCBI Official Synonym Symbols:	CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
NCBI Protein Information:	granzyme H
UniProt Protein Name:	Granzyme H
UniProt Synonym Protein Names:	CCP-X; Cathepsin G-like 2; CTSGL2; Cytotoxic T-lymphocyte proteinase; Cytotoxic serine protease C; CSP-C
Protein Family:	Granzyme
UniProt Gene Name:	GZMH
UniProt Entry Name:	GRAH_HUMAN

Western blot analysis of extracts of various cell lines, using GZMH Antibody (CAB6154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

Antibodies

GZMH Antibody (PACO04020)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)