Metabolism Antibodies 1

Anti-GYG1 Antibody (CAB10218)

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SKU:
CAB10218
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-GYG1 Antibody
Antibody SKU:CAB10218
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 234-333 of human GYG1 (NP_001171649.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, SW480, Mouse skeletal muscle, Mouse kidney, Mouse liver, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 234-333 of human GYG1 (NP_001171649.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EAHD PNMT HPEF LILW WNIF TTNV LPLL QQFG LVKD TCSY VNVE DVSG AISH LSLG EIPA MAQP FVSS EERK ERWE QGQA DYMG ADSF DNIK RKLD TYLQ
Gene ID:2992
Uniprot:P46976
Cellular Location:
Calculated MW:31kDa/37kDa/39kDa
Observed MW:39kDa
Synonyms:GYG1, GSD15, GYG
Background:This gene encodes a member of the glycogenin family. Glycogenin is a glycosyltransferase that catalyzes the formation of a short glucose polymer from uridine diphosphate glucose in an autoglucosylation reaction. This reaction is followed by elongation and branching of the polymer, catalyzed by glycogen synthase and branching enzyme, to form glycogen. This gene is expressed in muscle and other tissues. Mutations in this gene result in glycogen storage disease XV. This gene has pseudogenes on chromosomes 1, 8 and 13 respectively. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:glycogenin: Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Defects in GYG1 are the cause of glycogen storage disease type 15 (GSD15). It is a metabolic disorder resulting in muscle weakness, associated with the glycogen depletion in skeletal muscle, and cardiac arrhythmia, associated with the accumulation of abnormal storage material in the heart. The skeletal muscle shows a marked predominance of slow-twitch, oxidative muscle fibers and mitochondrial proliferation. Belongs to the glycosyltransferase 8 family. Glycogenin subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.4.1.186; Transferase

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: cytosol; extracellular region; lysosomal lumen; membrane

Molecular Function:glycogenin glucosyltransferase activity; protein binding

Biological Process: glycogen biosynthetic process; glycogen catabolic process; neutrophil degranulation

NCBI Summary:This gene encodes a member of the glycogenin family. Glycogenin is a glycosyltransferase that catalyzes the formation of a short glucose polymer from uridine diphosphate glucose in an autoglucosylation reaction. This reaction is followed by elongation and branching of the polymer, catalyzed by glycogen synthase and branching enzyme, to form glycogen. This gene is expressed in muscle and other tissues. Mutations in this gene result in glycogen storage disease XV. This gene has pseudogenes on chromosomes 1, 8 and 13 respectively. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]
UniProt Code:P46976
NCBI GenInfo Identifier:13432151
NCBI Gene ID:2992
NCBI Accession:P46976.4
UniProt Secondary Accession:P46976,Q6FHZ1, Q9UNV0, D3DNH0, D3DNH1, D3DNH2,
UniProt Related Accession:P46976
Molecular Weight:39kDa
NCBI Full Name:Glycogenin-1
NCBI Synonym Full Names:glycogenin 1
NCBI Official Symbol:GYG1  
NCBI Official Synonym Symbols:GYG; GSD15  
NCBI Protein Information:glycogenin-1
UniProt Protein Name:Glycogenin-1
UniProt Gene Name:GYG1  
Anti-GYG1 Antibody (CAB10218)Western blot analysis of extracts of various cell lines, using GYG1 antibody (CAB10218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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