Metabolism Antibodies 1

Anti-GUCY2C Antibody (CAB10562)

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SKU:
CAB10562
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-GUCY2C Antibody
Antibody SKU:CAB10562
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-330 of human GUCY2C (NP_004954.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HT-29, SW480, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-330 of human GUCY2C (NP_004954.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SQVS QNCH NGSY EISV LMMG NSAF AEPL KNLE DAVN EGLE IVRG RLQN AGLN VTVN ATFM YSDG LIHN SGDC RSST CEGL DLLR KISN AQRM GCVL IGPS CTYS TFQM YLDT ELSY PMIS AGSF GLSC DYKE TLTR LMSP ARKL MYFL VNFW KTND LPFK TYSW STSY VYKN GTET EDCF WYLN ALEA SVSY FSHE LGFK VVLR QDKE FQDI LMDH NRKS NVII MCGG PEFL YKLK GDRA VAED IVII LVDL FNDQ YFED NVTA PDYM KNVL VLTL SPGN SLLN SSFS RNLS PTKR DFAL AYLN GIL
Gene ID:2984
Uniprot:P25092
Cellular Location:Cell membrane, Endoplasmic reticulum membrane, Single-pass type I membrane protein
Calculated MW:123kDa
Observed MW:123kDa
Synonyms:GUCY2C, DIAR6, GC-C, GUC2C, MECIL, MUCIL, STAR
Background:This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive).
UniProt Protein Function:GUCY2C: an intestinal receptor for the E.coli enterotoxin, and the paracrine hormones guanylin and uroguanylin. Regulates intestinal cell proliferation. Converts guanosine-5'-triphosphate to cyclic guanosine monophosphate (cGMP). Elevated intracellular cGMP modulates intestinal fluid-ion homeostasis and differentiation of crypt enterocytes. cGMP inhibits colonic cell proliferation by inducing p21 and cell cycle arrest. mice lacking GC-C display increased cell proliferation in colonic crypts. It functions as a tumor suppressor; its loss disrupts intestinal homeostasis and promotes tumorigenesis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
UniProt Protein Details:

Protein type:EC 4.6.1.2; Guanylyl cyclase; Kinase, protein; Lyase; Membrane protein, integral; Nucleotide Metabolism - purine; Protein kinase, RGC; Protein kinase, dual-specificity (receptor); RGC family; RGC group; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: guanylate cyclase complex, soluble; plasma membrane

Molecular Function:adenylate cyclase activity; guanylate cyclase activity; protein binding

Biological Process: cGMP biosynthetic process; digestion; receptor guanylyl cyclase signaling pathway

Disease: Diarrhea 6; Meconium Ileus

NCBI Summary:This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive). [provided by RefSeq, Nov 2016]
UniProt Code:P25092
NCBI GenInfo Identifier:311033390
NCBI Gene ID:2984
NCBI Accession:P25092.2
UniProt Secondary Accession:P25092,B2RMY6,
UniProt Related Accession:P25092
Molecular Weight:123kDa
NCBI Full Name:Heat-stable enterotoxin receptor
NCBI Synonym Full Names:guanylate cyclase 2C
NCBI Official Symbol:GUCY2C  
NCBI Official Synonym Symbols:GC-C; STAR; DIAR6; GUC2C; MECIL; MUCIL  
NCBI Protein Information:heat-stable enterotoxin receptor
UniProt Protein Name:Heat-stable enterotoxin receptor
UniProt Synonym Protein Names:Guanylyl cyclase C; GC-C; Intestinal guanylate cyclase
Protein Family:Heat-stable enterotoxin receptor
UniProt Gene Name:GUCY2C  
Anti-GUCY2C Antibody (CAB10562)Western blot analysis of extracts of various cell lines, using GUCY2C antibody (CAB10562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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