Antibody Name:	Anti-GUCY1A3 Antibody
Antibody SKU:	CAB12710
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GUCY1A3 (NP_000847.2).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:3000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A-549, 293T, Mouse kidney, Mouse brain, Rat kidney, Rat spleen, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GUCY1A3 (NP_000847.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFCT KLKD LKIT GECP FSLL APGQ VPNE SSEE AAGS SESC KATV PICQ DIPE KNIQ ESLP QRKT SRSR VYLH TLAE SICK LIFP EFER LNVA LQRT LAKH KIKE SRKS LERE DFEK TIAE
Gene ID:	2982
Uniprot:	Q02108
Cellular Location:	Cytoplasm
Calculated MW:	70kDa/77kDa
Observed MW:	77kDa

Synonyms:

GUCY1A3, GC-SA3, GUC1A3, GUCA3, GUCSA3, GUCY1A1, MYMY6

Background:

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3', 5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.

UniProt Protein Function:	GUCY1A3: Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Protein Details:	Protein type:EC 4.6.1.2; Guanylyl cyclase; Lyase; Nucleotide Metabolism - purine; Receptor, misc. Chromosomal Location of Human Ortholog: 4q32.1 Cellular Component: guanylate cyclase complex, soluble; plasma membrane Molecular Function:adenylate cyclase activity; guanylate cyclase activity; protein binding; receptor activity Biological Process: blood circulation; cGMP biosynthetic process; nitric oxide mediated signal transduction Disease: Moyamoya Disease 6 With Achalasia
NCBI Summary:	Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:	Q02108
NCBI GenInfo Identifier:	7404351
NCBI Gene ID:	2982
NCBI Accession:	Q02108.2
UniProt Secondary Accession:	Q02108,O43843, Q8TAH3, D3DP19, D6RDW3,
UniProt Related Accession:	Q02108
Molecular Weight:	77kDa
NCBI Full Name:	Guanylate cyclase soluble subunit alpha-3
NCBI Synonym Full Names:	guanylate cyclase 1 soluble subunit alpha
NCBI Official Symbol:	GUCY1A3
NCBI Official Synonym Symbols:	GUCA3; MYMY6; GC-SA3; GUC1A3; GUCSA3; GUCY1A1
NCBI Protein Information:	guanylate cyclase soluble subunit alpha-3
UniProt Protein Name:	Guanylate cyclase soluble subunit alpha-3
UniProt Synonym Protein Names:	GCS-alpha-1; Soluble guanylate cyclase large subunit
Protein Family:	Guanylate cyclase soluble
UniProt Gene Name:	GUCY1A3

	Western blot analysis of extracts of various cell lines, using GUCY1A3 antibody (CAB12710) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of L929 cells using GUCY1A3 Rabbit pAb (CAB12710) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.