Epigenetics & Nuclear Signaling Antibodies 4
Anti-GTF3C4 Antibody (CAB9287)
(No reviews yet)
Write a Review
- SKU:
- CAB9287
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-GTF3C4 Antibody |
| Antibody SKU: | CAB9287 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 583-822 of human GTF3C4 (NP_036336.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | Jurkat, U-251MG, BT-474, HeLa, SW620 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 583-822 of human GTF3C4 (NP_036336.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ILYQ SMQK TPSE ALWK PTHE DSKI LLVD SPGM GNAD DEQQ EEGT SSKQ VVKQ GLQE RSKE GDVE EPTD DSLP TTGD AGGR EPME EKLL EIQG KIEA VEMH LTRE HMKR VLGE VYLH TWIT ENTS IPTR GLCN FLMS DEEY DDRT ARVL IGHI SKKM NKQT FPEH CSLC KEIL PFTD RKQA VCSN GHIW LRCF LTYQ SCQS LIYR RCLL HDSI ARHP APED PDWI KRLL QSPC PFCD SPVF |
| Gene ID: | 9329 |
| Uniprot: | Q9UKN8 |
| Cellular Location: | Nucleus |
| Calculated MW: | 91kDa |
| Observed MW: | 95kDa |
| Synonyms: | GTF3C4, KAT12, TF3C-delta, TFIII90, TFIIIC290, TFIIIC90, TFIIICDELTA |
| Background: |
| UniProt Protein Function: | GTF3C4: Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box. Belongs to the TFIIIC subunit 4 family. |
| UniProt Protein Details: | Protein type:EC 2.3.1.48; Acetyltransferase Chromosomal Location of Human Ortholog: 9q34.13 Cellular Component: nucleoplasm; transcription factor TFIIIC complex Molecular Function:DNA binding; enzyme activator activity; protein binding Biological Process: 5S class rRNA transcription; transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; transcription, DNA-dependent; tRNA transcription from RNA polymerase III promoter |
| UniProt Code: | Q9UKN8 |
| NCBI GenInfo Identifier: | 212276467 |
| NCBI Gene ID: | 9329 |
| NCBI Accession: | Q9UKN8.2 |
| UniProt Secondary Accession: | Q9UKN8,Q5VZJ7, |
| UniProt Related Accession: | Q9UKN8 |
| Molecular Weight: | 91,982 Da |
| NCBI Full Name: | General transcription factor 3C polypeptide 4 |
| NCBI Synonym Full Names: | general transcription factor IIIC subunit 4 |
| NCBI Official Symbol: | GTF3C4 |
| NCBI Official Synonym Symbols: | KAT12; TFIII90; TFIIIC90; TFIIIC290; TF3C-delta; TFIIICDELTA |
| NCBI Protein Information: | general transcription factor 3C polypeptide 4 |
| UniProt Protein Name: | General transcription factor 3C polypeptide 4 |
| UniProt Synonym Protein Names: | TF3C-delta; Transcription factor IIIC 90 kDa subunit; TFIIIC 90 kDa subunit; TFIIIC90; Transcription factor IIIC subunit delta |
| Protein Family: | General transcription factor 3C polypeptide |
| UniProt Gene Name: | GTF3C4 |
| UniProt Entry Name: | TF3C4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GTF3C4 antibody (CAB9287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using Anti-GTF3C4 Polyclonal Antibody (CAB9287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using Anti-GTF3C4 Polyclonal Antibody (CAB9287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
View AllClose
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human anti-ASGPR antibody(anti asialoglyco protein receptor antibody)ELISA Kit
OverviewProduct Name:Human anti-ASGPR antibody(anti asialoglyco protein receptor antibody)ELISA KitProduct Code:AEFI00360Size:96TAlias:a