Name: Anti-GTF3A Antibody (CAB8426)
Brand: Cell Biology Antibodies 12
SKU: CAB8426
Availability: OutOfStock

Antibody Name:	Anti-GTF3A Antibody
Antibody SKU:	CAB8426
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF3A (NP_002088.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	SW480

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF3A (NP_002088.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDPP AVVA ESVS SLTI ADAF IAAG ESSA PTPP RPAL PRRF ICSF PDCS ANYS KAWK LDAH LCKH TGER PFVC DYEG CGKA FIRD YHLS RHIL THTG EKPF
Gene ID:	2971
Uniprot:	Q92664
Cellular Location:	Nucleus
Calculated MW:	38kDa/41kDa
Observed MW:	38kDa

Synonyms:

GTF3A, AP2, TFIIIA

Background:

The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein.

UniProt Protein Function:	GTF3A: Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription initiation complex; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 13q12.3-q13.1 Cellular Component: nucleoplasm; nucleus Biological Process: rRNA transcription; transcription from RNA polymerase III promoter
NCBI Summary:	The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92664
NCBI GenInfo Identifier:	172045838
NCBI Gene ID:	2971
NCBI Accession:	Q92664.3
UniProt Secondary Accession:	Q92664,Q12963, Q13097, B7ZBK5,
UniProt Related Accession:	Q92664
Molecular Weight:	38,779 Da
NCBI Full Name:	Transcription factor IIIA
NCBI Synonym Full Names:	general transcription factor IIIA
NCBI Official Symbol:	GTF3A
NCBI Official Synonym Symbols:	AP2; TFIIIA
NCBI Protein Information:	transcription factor IIIA
UniProt Protein Name:	Transcription factor IIIA
UniProt Gene Name:	GTF3A
UniProt Entry Name:	TF3A_HUMAN

Anti-GTF3A Polyclonal Antibody (CAB8426)

Western blot analysis of extracts of SW480 cells, using GTF3A antibody (CAB8426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

GTF3A Antibody (PACO04006)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)