Antibody Name:	Anti-GTF2H2 Antibody
Antibody SKU:	CAB5317
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF2H2 (NP_001506.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, Mouse thymus, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF2H2 (NP_001506.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDEE PERT KRWE GGYE RTWE ILKE DESG SLKA TIED ILFK AKRK RVFE HHGQ VRLG MMRH LYVV VDGS RTME DQDL KPNR LTCT LKLL EYFV EEYF DQNP
Gene ID:	2966
Uniprot:	Q13888
Cellular Location:	Nucleus
Calculated MW:	44kDa
Observed MW:	44kDa

Synonyms:

GTF2H2, BTF2, BTF2P44, T-BTF2P44, TFIIH, p44

Background:

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene.

UniProt Protein Function:	Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.
NCBI Summary:	This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13888
NCBI GenInfo Identifier:	17380326
NCBI Gene ID:	2966
NCBI Accession:	Q13888.1
UniProt Secondary Accession:	Q13888,Q15570, Q15571, Q9BS41,
UniProt Related Accession:	Q13888
Molecular Weight:	44,419 Da
NCBI Full Name:	General transcription factor IIH subunit 2
NCBI Synonym Full Names:	general transcription factor IIH subunit 2
NCBI Official Symbol:	GTF2H2
NCBI Official Synonym Symbols:	p44; BTF2; TFIIH; BTF2P44; T-BTF2P44
NCBI Protein Information:	general transcription factor IIH subunit 2
UniProt Protein Name:	General transcription factor IIH subunit 2
UniProt Synonym Protein Names:	Basic transcription factor 2 44 kDa subunit; BTF2 p44; General transcription factor IIH polypeptide 2; TFIIH basal transcription factor complex p44 subunit
UniProt Gene Name:	GTF2H2
UniProt Entry Name:	TF2H2_HUMAN

	Western blot analysis of extracts of various cell lines, using GTF2H2 Antibody (CAB5317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat lung using GTF2H2 antibody (CAB5317) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using GTF2H2 antibody (CAB5317) at dilution of 1:100 (40x lens).