Epigenetics & Nuclear Signaling Antibodies 4
Anti-GTF2H1 Antibody (CAB6814)
- SKU:
- CAB6814
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-GTF2H1 Antibody |
| Antibody SKU: | CAB6814 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-425 of human GTF2H1 (NP_001135779.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, A-549, MCF-7, NIH/3T3, Mouse testis, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-425 of human GTF2H1 (NP_001135779.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATS SEEV LLIV KKVR QKKQ DGAL YLMA ERIA WAPE GKDR FTIS HMYA DIKC QKIS PEGK AKIQ LQLV LHAG DTTN FHFS NEST AVKE RDAV KDLL QQLL PKFK RKAN KELE EKNR MLQE DPVL FQLY KDLV VSQV ISAE EFWA NRLN VNAT DSSS TSNH KQDV GISA AFLA DVRP QTDG CNGL RYNL TSDI IESI FRTY PAVK MKYA ENVP HNMT EKEF WTRF FQSH YFHR DRLN TGSK DLFA ECAK IDEK GLKT MVSL GVKN PLLD LTAL EDKP LDEG YGIS SVPS ASNS KSIK ENSN AAII KRFN HHSA MVLA AGLR KQEA QNEQ TSEP SNMD GNSG DADC FQPA VKRA KLQE SIEY EDLG KNNS VKTI ALNL KKSD RYYH GPTP IQSL QYAT SQDI INSF QSIR QEME AYTP KLTQ VLSS S |
| Gene ID: | 2965 |
| Uniprot: | P32780 |
| Cellular Location: | Nucleus |
| Calculated MW: | 48kDa/62kDa |
| Observed MW: | 65kDa |
| Synonyms: | GTF2H1, BTF2, P62, TFB1, TFIIH |
| Background: |
| UniProt Protein Function: | GTF2H1: Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. |
| UniProt Protein Details: | Protein type:Transcription initiation complex Chromosomal Location of Human Ortholog: 11p15.1-p14 Cellular Component: holo TFIIH complex; nucleoplasm Molecular Function:chromatin binding; DNA-dependent ATPase activity; protein binding; protein kinase activity; RNA polymerase subunit kinase activity Biological Process: DNA repair; gene expression; mRNA capping; negative regulation of gene expression, epigenetic; nucleotide-excision repair; nucleotide-excision repair, DNA incision; positive regulation of transcription from RNA polymerase II promoter; positive regulation of viral transcription; protein amino acid phosphorylation; regulation of cyclin-dependent protein kinase activity; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; viral reproduction |
| UniProt Code: | P32780 |
| NCBI GenInfo Identifier: | 416727 |
| NCBI Gene ID: | 2965 |
| NCBI Accession: | P32780.1 |
| UniProt Secondary Accession: | P32780,Q6I9Y7, Q9H5K5, Q9NQD9, B3KXE0, D3DQY2, |
| UniProt Related Accession: | P32780,AAB23294 |
| Molecular Weight: | 48,738 Da |
| NCBI Full Name: | General transcription factor IIH subunit 1 |
| NCBI Synonym Full Names: | general transcription factor IIH subunit 1 |
| NCBI Official Symbol: | GTF2H1 |
| NCBI Official Synonym Symbols: | P62; BTF2; TFB1; TFIIH |
| NCBI Protein Information: | general transcription factor IIH subunit 1 |
| UniProt Protein Name: | General transcription factor IIH subunit 1 |
| UniProt Synonym Protein Names: | Basic transcription factor 2 62 kDa subunit; BTF2 p62; General transcription factor IIH polypeptide 1; TFIIH basal transcription factor complex p62 subunit |
| Protein Family: | General transcription factor |
| UniProt Gene Name: | GTF2H1 |
| UniProt Entry Name: | TF2H1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GTF2H1 antibody (CAB6814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U-2 OS cells using GTF2H1 Rabbit pAb (CAB6814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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