Epigenetics & Nuclear Signaling Antibodies 2
Anti-GTF2F2 Antibody (CAB5826)
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- SKU:
- CAB5826
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-GTF2F2 Antibody |
| Antibody SKU: | CAB5826 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human GTF2F2 (NP_004119.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | SW480, 293T, HepG2, HL-60, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human GTF2F2 (NP_004119.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAER GELD LTGA KQNT GVWL VKVP KYLS QQWA KASG RGEV GKLR IAKT QGRT EVSF TLNE DLAN IHDI GGKP ASVS APRE HPFV LQSV GGQT LTVF TESS SDKL SLEG IVVQ RAEC RPAA SENY MRLK RLQI EESS KPVR LSQQ LDKV VTTN YKPV ANHQ YNIE YERK KKED GKRA RADK QHVL DMLF SAFE KHQY YNLK DLVD ITKQ PVVY LKEI LKEI GVQN VKGI HKNT WELK PEYR HYQG EEKS D |
| Gene ID: | 2963 |
| Uniprot: | P13984 |
| Cellular Location: | Nucleus |
| Calculated MW: | 28kDa |
| Observed MW: | 29kDa |
| Synonyms: | GTF2F2, BTF4, RAP30, TF2F2, TFIIF |
| Background: |
| UniProt Protein Function: | GTF2F2: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. Belongs to the TFIIF beta subunit family. |
| UniProt Protein Details: | Protein type:Helicase; EC 3.6.4.12; Nuclear receptor co-regulator; Transcription initiation complex Chromosomal Location of Human Ortholog: 13q14 Cellular Component: nucleoplasm; microtubule cytoskeleton; transcription factor TFIIF complex; nucleus Molecular Function:protein binding; DNA binding; ATP-dependent helicase activity; ATP binding Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; regulation of transcription, DNA-dependent; positive regulation of viral transcription; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression |
| UniProt Code: | P13984 |
| NCBI GenInfo Identifier: | 464519 |
| NCBI Gene ID: | 2963 |
| NCBI Accession: | P13984.2 |
| UniProt Secondary Accession: | P13984,Q5W0H3, A6NNS5, |
| UniProt Related Accession: | P13984 |
| Molecular Weight: | 249 |
| NCBI Full Name: | General transcription factor IIF subunit 2 |
| NCBI Synonym Full Names: | general transcription factor IIF, polypeptide 2, 30kDa |
| NCBI Official Symbol: | GTF2F2 |
| NCBI Official Synonym Symbols: | BTF4; RAP30; TF2F2; TFIIF |
| NCBI Protein Information: | general transcription factor IIF subunit 2; TFIIF-beta; ATP-dependent helicase GTF2F2; transcription initiation factor RAP30; general transcription factor IIF 30 kDa subunit; transcription initiation factor IIF subunit beta |
| UniProt Protein Name: | General transcription factor IIF subunit 2 |
| UniProt Synonym Protein Names: | ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30 |
| Protein Family: | General transcription factor |
| UniProt Gene Name: | GTF2F2 |
| UniProt Entry Name: | T2FB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GTF2F2 antibody (CAB5826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of A549 cells using GTF2F2 antibody (CAB5826). Blue: DAPI for nuclear staining. |
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