Epigenetics & Nuclear Signaling Antibodies 3
Anti-GTF2F1 Antibody (CAB2489)
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- SKU:
- CAB2489
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-GTF2F1 Antibody |
| Antibody SKU: | CAB2489 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-505 of human GTF2F1 (NP_002087.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | 293T, K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-505 of human GTF2F1 (NP_002087.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DSSE ESDS SEES DIDS EASS ALFM AKKK TPPK RERK PSGG SSRG NSRP GTPS AEGG STSS TLRA AASK LEQG KRVS EMPA AKRL RLDT GPQS LSGK STPQ PPSG KTTP NSGD VQVT EDAV RRYL TRKP MTTK DLLK KFQT KKTG LSSE QTVN VLAQ ILKR LNPE RK |
| Gene ID: | 2962 |
| Uniprot: | P35269 |
| Cellular Location: | Nucleus |
| Calculated MW: | 58kDa |
| Observed MW: | 74kDa |
| Synonyms: | BTF4, RAP74, TF2F1, TFIIF, GTF2F1 |
| Background: |
| UniProt Protein Function: | GTF2F1: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the TFIIF alpha subunit family. |
| UniProt Protein Details: | Protein type:Kinase, protein; EC 2.7.11.1; Transcription initiation complex; ATYPICAL group; GTF2F1 family Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; transcription factor TFIIF complex; cell junction; nucleus Molecular Function:protein binding; DNA binding; phosphatase activator activity; transcription coactivator activity; transcription factor binding; catalytic activity Biological Process: positive regulation of catalytic activity; transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of viral transcription; RNA splicing; response to virus; RNA elongation from RNA polymerase II promoter; gene expression |
| UniProt Code: | P35269 |
| NCBI GenInfo Identifier: | 46397744 |
| NCBI Gene ID: | 2962 |
| NCBI Accession: | P35269.2 |
| UniProt Secondary Accession: | P35269,Q9BWN0, B2RCS0, |
| UniProt Related Accession: | P35269 |
| Molecular Weight: | 517 |
| NCBI Full Name: | General transcription factor IIF subunit 1 |
| NCBI Synonym Full Names: | general transcription factor IIF, polypeptide 1, 74kDa |
| NCBI Official Symbol: | GTF2F1 |
| NCBI Official Synonym Symbols: | BTF4; RAP74; TF2F1; TFIIF |
| NCBI Protein Information: | general transcription factor IIF subunit 1; TFIIF-alpha; transcription initiation factor RAP74; general transcription factor IIF 74 kDa subunit; transcription initiation factor IIF subunit alpha |
| UniProt Protein Name: | General transcription factor IIF subunit 1 |
| UniProt Synonym Protein Names: | General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74 |
| Protein Family: | General transcription factor |
| UniProt Gene Name: | GTF2F1 |
| UniProt Entry Name: | T2FA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GTF2F1 antibody (CAB2489) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat liver using GTF2F1 antibody (CAB2489) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human thyroid cancer using GTF2F1 antibody (CAB2489) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human normal lung using GTF2F1 antibody (CAB2489) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using GTF2F1 antibody (CAB2489). |
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