Immunology Antibodies 1
Anti-GTF2E1 Antibody (CAB15275)
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- SKU:
- CAB15275
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-GTF2E1 Antibody |
| Antibody SKU: | CAB15275 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 230-439 of human GTF2E1 (NP_005504.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, A-431, Mouse brain, Mouse spleen, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 230-439 of human GTF2E1 (NP_005504.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LAGG HHRE AWAT KGPS YEDL YTQN VVIN MDDQ EDLH RASL EGKS AKER PIWL REST VQGA YGSE DMKE GGID MDAF QERE EGHA GPDD NEEV MRAL LIHE KKTS SAMA GSVG AAAP VTAA NGSD SESE TSES DDDS PPRP AAVA VHKR EEDE EEDD EFEE VADD PIVM VAGR PFSY SEVS QRPE LVAQ MTPE EKEA YIAM GQRM FEDL FE |
| Gene ID: | 2960 |
| Uniprot: | P29083 |
| Cellular Location: | Nucleus |
| Calculated MW: | 49kDa |
| Observed MW: | 60kDa |
| Synonyms: | GTF2E1, FE, TF2E1, TFIIE-A |
| Background: |
| UniProt Protein Function: | GTF2E1: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Belongs to the TFIIE alpha subunit family. |
| UniProt Protein Details: | Protein type:Transcription initiation complex Chromosomal Location of Human Ortholog: 3q21-q24 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter |
| UniProt Code: | P29083 |
| NCBI GenInfo Identifier: | 116242812 |
| NCBI Gene ID: | 2960 |
| NCBI Accession: | P29083.2 |
| UniProt Secondary Accession: | P29083,Q16103, |
| UniProt Related Accession: | P29083 |
| Molecular Weight: | 49,452 Da |
| NCBI Full Name: | General transcription factor IIE subunit 1 |
| NCBI Synonym Full Names: | general transcription factor IIE subunit 1 |
| NCBI Official Symbol: | GTF2E1 |
| NCBI Official Synonym Symbols: | FE; TF2E1; TFIIE-A |
| NCBI Protein Information: | general transcription factor IIE subunit 1 |
| UniProt Protein Name: | General transcription factor IIE subunit 1 |
| UniProt Synonym Protein Names: | General transcription factor IIE 56 kDa subunit; Transcription initiation factor IIE subunit alpha; TFIIE-alpha |
| Protein Family: | General transcription factor |
| UniProt Gene Name: | GTF2E1 |
| UniProt Entry Name: | T2EA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GTF2E1 antibody (CAB15275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5S. |
 | Immunofluorescence analysis of U-2 OS cells using GTF2E1 Polyclonal Antibody (CAB15275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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