Immunology Antibodies 2

Anti-GTF2A2 Antibody (CAB7680)

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SKU:
CAB7680
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-GTF2A2 Antibody
Antibody SKU:CAB7680
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-109 of human GTF2A2 (NP_004483.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:NCI-H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-109 of human GTF2A2 (NP_004483.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAYQ LYRN TTLG NSLQ ESLD ELIQ SQQI TPQL ALQV LLQF DKAI NAAL AQRV RNRV NFRG SLNT YRFC DNVW TFVL NDVE FREV TELI KVDK VKIV ACDG KNTG SNTT E
Gene ID:2958
Uniprot:P52657
Cellular Location:Nucleus
Calculated MW:12kDa
Observed MW:12kDa
Synonyms:GTF2A2, HsT18745, T18745, TF2A2, TFIIA, TFIIA-12, TFIIA-gamma, TFIIAS
Background:
UniProt Protein Function:GTF2A2: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Belongs to the TFIIA subunit 2 family.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: cell junction; nucleoplasm; transcription factor TFIIA complex

Molecular Function:protein binding; protein heterodimerization activity; protein homodimerization activity; TATA-binding protein binding; transcription coactivator activity; transcription factor binding

Biological Process: positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcriptional preinitiation complex assembly; viral reproduction

NCBI Summary:Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008]
UniProt Code:P52657
NCBI GenInfo Identifier:49457380
NCBI Gene ID:2958
NCBI Accession:CAG46989.1
UniProt Related Accession:P52657
Molecular Weight:
NCBI Full Name:GTF2A2, partial
NCBI Synonym Full Names:general transcription factor IIA subunit 2
NCBI Official Symbol:GTF2A2  
NCBI Official Synonym Symbols:TF2A2; TFIIA; T18745; TFIIAS; HsT18745; TFIIA-12; TFIIA-gamma  
NCBI Protein Information:transcription initiation factor IIA subunit 2
UniProt Protein Name:Transcription initiation factor IIA subunit 2
UniProt Synonym Protein Names:General transcription factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; Transcription initiation factor IIA gamma chain; TFIIA-gamma
UniProt Gene Name:GTF2A2  
UniProt Entry Name:T2AG_HUMAN
Anti-GTF2A2 Antibody (CAB7680)Western blot analysis of extracts of NCI-H460 cells, using GTF2A2 antibody (CAB7680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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