Epigenetics & Nuclear Signaling Antibodies 3
Anti-GTF2A1 Antibody (CAB5345)
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- SKU:
- CAB5345
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-GTF2A1 Antibody |
| Antibody SKU: | CAB5345 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human GTF2A1 (NP_056943.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | BT-474, Raji |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human GTF2A1 (NP_056943.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MANS ANTN TVPK LYRS VIED VIND VRDI FLDD GVDE QVLM ELKT LWEN KLMQ SRAV DGFH SEEQ QLLL QVQQ QHQP QQQQ HHHH HHHQ QAQP QQTV PQQA QTQQ VLIP ASQQ ATAP QVIV PDSK LIQH MNAS NMSA AATA ATLA LPAG VTPV QQIL TNSG QLLQ VVRA ANGA QYIF QPQQ SVVL QQQV IPQM QPGG VQAP VIQQ VLAP LPGG ISPQ TGVI IQPQ QILF TGNK TQVI PTTV AAPT PAQA QITA TGQQ QPQA QPAQ TQAP LV |
| Gene ID: | 2957 |
| Uniprot: | P52655 |
| Cellular Location: | Nucleus |
| Calculated MW: | 37kDa/41kDa |
| Observed MW: | 37kDa |
| Synonyms: | GTF2A1, TF2A1, TFIIA, TFIIA-42, TFIIAL |
| Background: |
| UniProt Protein Function: | GTF2A1: a general transcription factor that plays an important role in transcriptional activation. A component of the transcription machinery of RNA polymerase II. Phosphorylated by Taf(II) 250 on serines that regulate TBP binding. Interacts with TBP (the TATA-binding protein). Two isoforms, 42 kDa and 37 kDa, are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 14q31.1 Cellular Component: cytoplasm; nucleoplasm; transcription factor TFIIA complex Molecular Function:DNA binding; protein binding; protein heterodimerization activity; TATA-binding protein binding; transcription coactivator activity; transcription factor binding Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010] |
| UniProt Code: | P52655 |
| NCBI GenInfo Identifier: | 1711663 |
| NCBI Gene ID: | 2957 |
| NCBI Accession: | P52655.1 |
| UniProt Secondary Accession: | P52655,Q3KNQ9, |
| UniProt Related Accession: | P52655 |
| Molecular Weight: | 37,152 Da |
| NCBI Full Name: | Transcription initiation factor IIA subunit 1 |
| NCBI Synonym Full Names: | general transcription factor IIA subunit 1 |
| NCBI Official Symbol: | GTF2A1 |
| NCBI Official Synonym Symbols: | TF2A1; TFIIA; TFIIAL; TFIIA-42 |
| NCBI Protein Information: | transcription initiation factor IIA subunit 1 |
| UniProt Protein Name: | Transcription initiation factor IIA subunit 1 |
| UniProt Synonym Protein Names: | General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42 |
| Protein Family: | Transcription initiation factor |
| UniProt Gene Name: | GTF2A1 |
| UniProt Entry Name: | TF2AA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GTF2A1 antibody (CAB5345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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