Metabolism Antibodies 2

Anti-GSTZ1 Antibody (CAB6129)

(No reviews yet) Write a Review
SKU:
CAB6129
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-GSTZ1 Antibody
Antibody SKU:CAB6129
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human GSTZ1 (NP_665877.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse kidney, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human GSTZ1 (NP_665877.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQAG KPIL YSYF RSSC SWRV RIAL ALKG IDYE TVPI NLIK DGGQ QFSK DFQA LNPM KQVP TLKI DGIT IHQS LAII EYLE EMRP TPRL LPQD PKKR ASVR MISD LIAG GIQP LQNL SVLK QVGE EMQL TWAQ NAIT CGFN ALEQ ILQS TAGI YCVG DEVT MADL CLVP QVAN AERF KVDL TPYP TISS INKR LLVL EAFQ VSHP CRQP DTPT ELRA
Gene ID:2954
Uniprot:O43708
Cellular Location:Cytoplasm
Calculated MW:17kDa/19kDa/24kDa
Observed MW:24KDa
Synonyms:GSTZ1, GSTZ1-1, MAAI, MAI
Background:This gene is a member of the glutathione S-transferase (GSTs) super-family which encodes multifunctional enzymes important in the detoxification of electrophilic molecules, including carcinogens, mutagens, and several therapeutic drugs, by conjugation with glutathione. This enzyme catalyzes the conversion of maleylacetoacetate to fumarylacetoacatate, which is one of the steps in the phenylalanine/tyrosine degradation pathway. Deficiency of a similar gene in mouse causes oxidative stress. Several transcript variants of this gene encode multiple protein isoforms.
UniProt Protein Function:GSTZ1: Bifunctional enzyme showing minimal glutathione- conjugating activity with ethacrynic acid and 7-chloro-4- nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T- butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. Belongs to the GST superfamily. Zeta family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Xenobiotic Metabolism - metabolism by cytochrome P450; EC 5.2.1.2; Isomerase; Transferase; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tyrosine; Mitochondrial; Oxidoreductase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: mitochondrion; cytosol

Molecular Function:protein binding; protein homodimerization activity; glutathione transferase activity; maleylacetoacetate isomerase activity; glutathione peroxidase activity

Biological Process: L-phenylalanine catabolic process; glutathione metabolic process; tyrosine catabolic process; xenobiotic metabolic process

NCBI Summary:This gene is a member of the glutathione S-transferase (GSTs) super-family which encodes multifunctional enzymes important in the detoxification of electrophilic molecules, including carcinogens, mutagens, and several therapeutic drugs, by conjugation with glutathione. This enzyme also plays a significant role in the catabolism of phenylalanine and tyrosine. Thus defects in this enzyme may lead to severe metabolic disorders including alkaptonuria, phenylketonuria and tyrosinaemia. Several transcript variants of this gene encode multiple protein isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:O43708
NCBI GenInfo Identifier:269849671
NCBI Gene ID:2954
NCBI Accession:O43708.3
UniProt Secondary Accession:O43708,O15308, O75430, Q6IB17, Q7Z610, Q9BV63, A6NED0 A6NNB8, A8MWD7, B2RCK3,
UniProt Related Accession:O43708
Molecular Weight:216
NCBI Full Name:Maleylacetoacetate isomerase
NCBI Synonym Full Names:glutathione S-transferase zeta 1
NCBI Official Symbol:GSTZ1  
NCBI Official Synonym Symbols:MAI; MAAI; GSTZ1-1  
NCBI Protein Information:maleylacetoacetate isomerase; maleylacetone isomerase; glutathione S-aryltransferase; glutathione S-alkyltransferase; glutathione transferase zeta 1; glutathione S-aralkyltransferase; S-(hydroxyalkyl)glutathione lyase
UniProt Protein Name:Maleylacetoacetate isomerase
UniProt Synonym Protein Names:GSTZ1-1; Glutathione S-transferase zeta 1 (EC:2.5.1.18)
Protein Family:Glutathione S-transferase
UniProt Gene Name:GSTZ1  
UniProt Entry Name:MAAI_HUMAN
Anti-GSTZ1 Antibody (CAB6129)Western blot analysis of extracts of various cell lines, using GSTZ1 antibody (CAB6129) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
View AllClose

0 Reviews

View AllClose

Related Products

Human MAA/GSTZ1 Recombinant Protein (RPES4159)

Human MAA/GSTZ1 Recombinant Protein (RPES4159)

Metabolism Recombinant Proteins

system_update_altDatasheetHuman MAA/GSTZ1 Recombinant Protein Maleylacetoacetate Isomerase (MAAI) belongs to the Glutathione S-Transferase super-family. MAAI encodes multifunctional enzymes in th