Antibody Name:	Anti-GSTT2B Antibody
Antibody SKU:	CAB15583
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 115-244 of human GSTT2B (NP_001074312.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse lung, Rat brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 115-244 of human GSTT2B (NP_001074312.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WVQV LGPL IGVQ VPEE KVER NRTA MDQA LQWL EDKF LGDR PFLA GQQV TLAD LMAL EELM QPVA LGYE LFEG RPRL AAWR GRVE AFLG AELC QEAH SIIL SILE QAAK KTLP TPSP EAYQ AMLL RIAR IP
Gene ID:	653689
Uniprot:	P0CG30
Cellular Location:	Cytoplasm
Calculated MW:	27kDa
Observed MW:	27kDa

Synonyms:

GSTT2B, GSTT2P

Background:

The protein encoded by this gene, glutathione S-transferase (GST) theta 2B (GSTT2B), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT2 and GSTT2B are nearly identical to each other, and share 55% amino acid identity with GSTT1. All three genes may play a role in human carcinogenesis. The GSTT2B gene is a pseudogene in some populations.

UniProt Protein Function:	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a sulfatase activity.
NCBI Summary:	The protein encoded by this gene, glutathione S-transferase (GST) theta 2 (GSTT2), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT2 and GSTT2B are nearly identical to each other, and share 55% amino acid identity with GSTT1. All three genes may play a role in human carcinogenesis. The GSTT2 gene is a pseudogene in some populations. [provided by RefSeq, Sep 2015]
UniProt Code:	P0CG30
NCBI GenInfo Identifier:	702760663
NCBI Gene ID:	2953
NCBI Accession:	NP_000845.2
UniProt Secondary Accession:	P0CG30,O60665, P30712, Q6IPV7, Q9HD76,
UniProt Related Accession:	P0CG29
Molecular Weight:	27,507 Da
NCBI Full Name:	glutathione S-transferase theta-2 isoform a
NCBI Synonym Full Names:	glutathione S-transferase theta 2 (gene/pseudogene)
NCBI Official Symbol:	GSTT2
NCBI Protein Information:	glutathione S-transferase theta-2
UniProt Protein Name:	Glutathione S-transferase theta-2B
UniProt Synonym Protein Names:	GST class-theta-2; Glutathione S-transferase theta-2
Protein Family:	Glutathione S-transferase
UniProt Gene Name:	GSTT2B

	Western blot analysis of extracts of various cell lines, using GSTT2B antibody (CAB15583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
	Immunofluorescence analysis of HeLa cells using GSTT2B Rabbit pAb (CAB15583) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using GSTT2B Rabbit pAb (CAB15583) at dilution of 1:100. Blue: DAPI for nuclear staining.