Cell Biology Antibodies 9

Anti-GSTP1 Antibody (CAB5691)

(No reviews yet) Write a Review
SKU:
CAB5691
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-GSTP1 Antibody
Antibody SKU:CAB5691
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human GSTP1 (NP_000843.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:SW620, HL-60, HT-29, 293T, U-251MG, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human GSTP1 (NP_000843.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPPY TVVY FPVR GRCA ALRM LLAD QGQS WKEE VVTV ETWQ EGSL KASC LYGQ LPKF QDGD LTLY QSNT ILRH LGRT LGLY GKDQ QEAA LVDM VNDG VEDL RCKY ISLI YTNY EAGK DDYV KALP GQLK PFET LLSQ NQGG KTFI VGDQ ISFA DYNL LDLL LIHE VLAP GCLD AFPL LSAY VGRL SARP KLKA FLAS PEYV NLPI NGNG KQ
Gene ID:2950
Uniprot:P09211
Cellular Location:Cytoplasm, Mitochondrion, Nucleus
Calculated MW:23kDa
Observed MW:25kDa
Synonyms:DFN7, FAEES3, GST3, GSTP, HEL-S-22, PI, GST3 / GSTP1, GSTP1
Background:Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
UniProt Protein Function:GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family.
UniProt Protein Details:

Protein type:Transferase; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; mitochondrion; cytoplasm; plasma membrane; intracellular; cytosol; nucleus; vesicle

Molecular Function:JUN kinase binding; protein binding; glutathione transferase activity; kinase regulator activity

Biological Process: negative regulation of MAP kinase activity; negative regulation of JNK activity; central nervous system development; negative regulation of MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of stress-activated MAPK cascade; regulation of stress-activated MAPK cascade; negative regulation of biosynthetic process; response to reactive oxygen species; glutathione metabolic process; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of fibroblast proliferation; positive regulation of superoxide release; xenobiotic metabolic process; negative regulation of protein kinase activity; negative regulation of apoptosis

NCBI Summary:Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
UniProt Code:P09211
NCBI GenInfo Identifier:121746
NCBI Gene ID:2950
NCBI Accession:P09211.2
UniProt Secondary Accession:P09211,O00460, Q15690, Q5TZY3,
UniProt Related Accession:P09211
Molecular Weight:23,356 Da
NCBI Full Name:Glutathione S-transferase P
NCBI Synonym Full Names:glutathione S-transferase pi 1
NCBI Official Symbol:GSTP1  
NCBI Official Synonym Symbols:PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22  
NCBI Protein Information:glutathione S-transferase P; GSTP1-1; GST class-pi; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III
UniProt Protein Name:Glutathione S-transferase P
UniProt Synonym Protein Names:GST class-pi; GSTP1-1
Protein Family:Glutathione S-transferase
UniProt Gene Name:GSTP1  
UniProt Entry Name:GSTP1_HUMAN
Anti-GSTP1 Antibody (CAB5691)Western blot analysis of extracts of various cell lines, using GSTP1 antibody (CAB5691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-GSTP1 Antibody (CAB5691)Immunohistochemistry of paraffin-embedded human liver cancer using GSTP1 antibody (CAB5691) at dilution of 1:200 (40x lens).
Anti-GSTP1 Antibody (CAB5691)Immunohistochemistry of paraffin-embedded mouse kidney using GSTP1 antibody (CAB5691) at dilution of 1:200 (40x lens).
Anti-GSTP1 Antibody (CAB5691)Immunofluorescence analysis of L929 cells using GSTP1 Rabbit pAb (CAB5691) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products