KO Validated Antibodies 2

Anti-GSTO1 Antibody (CAB19892)[KO Validated]

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SKU:
CAB19892
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GSTO1 Antibody [KO Validated]
Antibody SKU:CAB19892
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human GSTO1.
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human GSTO1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:9446
Uniprot:P78417
Cellular Location:Cytoplasm, cytosol
Calculated MW:23kDa/24kDa/27kDa
Observed MW:27KDa
Anti-GSTO1 Antibody [KO Validated] (CAB19892)Western blot analysis of extracts from normal (control) and GSTO1 knockout (KO) 293T cells, using GSTO1 antibody (CAB19892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:GSTO1, GSTO 1-1, GSTTLp28, HEL-S-21, P28, SPG-R, GSTO1-1
Background:The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:GSTO1: Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. Belongs to the GST superfamily. Omega family.
UniProt Protein Details:

Protein type:EC 1.8.5.1; Transferase; EC 1.20.4.2; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 10q25.1

Cellular Component: cytoplasm; cytosol

Molecular Function:glutathione dehydrogenase (ascorbate) activity; glutathione transferase activity; methylarsonate reductase activity; oxidoreductase activity; protein binding

Biological Process: glutathione metabolic process; L-ascorbic acid metabolic process; methylation; positive regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; xenobiotic catabolic process

NCBI Summary:The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:P78417
NCBI GenInfo Identifier:6016173
NCBI Gene ID:9446
NCBI Accession:P78417.2
UniProt Secondary Accession:P78417,Q5TA03, Q7Z3T2, D3DRA3, F5H7H0,
UniProt Related Accession:P78417
Molecular Weight:24,796 Da
NCBI Full Name:Glutathione S-transferase omega-1
NCBI Synonym Full Names:glutathione S-transferase omega 1
NCBI Official Symbol:GSTO1  
NCBI Official Synonym Symbols:P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21  
NCBI Protein Information:glutathione S-transferase omega-1
UniProt Protein Name:Glutathione S-transferase omega-1
UniProt Synonym Protein Names:Glutathione S-transferase omega 1-1; GSTO 1-1; Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1
Protein Family:Glutathione S-transferase
UniProt Gene Name:GSTO1  
UniProt Entry Name:GSTO1_HUMAN
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