Name: Anti-GSTM5 Antibody (CAB15677)
Brand: Cell Biology Antibodies 6
SKU: CAB15677
Availability: OutOfStock

Antibody Name:	Anti-GSTM5 Antibody
Antibody SKU:	CAB15677
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human GSTM5 (NP_000842.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Mouse, Rat
Positive Samples:	Mouse heart, Mouse kidney, Mouse liver, Rat testis, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human GSTM5 (NP_000842.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LENQ VMDN HMEL VRLC YDPD FEKL KPKY LEEL PEKL KLYS EFLG KRPW FAGD KITF VDFL AYDV LDMK RIFE PKCL DAFL NLKD FISR FEGL KKIS AYMK SSQF LRGL LFGK SATW NSK
Gene ID:	2949
Uniprot:	P46439
Cellular Location:	Cytoplasm
Calculated MW:	25kDa
Observed MW:	24-26kDa

Synonyms:

GSTM5, GSTM5-5, GTM5

Background:

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds.

UniProt Protein Function:	GSTM5: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family.
UniProt Protein Details:	Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18 Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: cytosol Molecular Function:glutathione transferase activity; protein binding Biological Process: glutathione metabolic process
NCBI Summary:	Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. [provided by RefSeq, Jul 2008]
UniProt Code:	P46439
NCBI GenInfo Identifier:	67476963
NCBI Gene ID:	2949
NCBI Accession:	P46439.3
UniProt Secondary Accession:	P46439,Q6PD78, A8K0V8,
UniProt Related Accession:	P46439
Molecular Weight:	25,675 Da
NCBI Full Name:	Glutathione S-transferase Mu 5
NCBI Synonym Full Names:	glutathione S-transferase mu 5
NCBI Official Symbol:	GSTM5
NCBI Official Synonym Symbols:	GTM5; GSTM5-5
NCBI Protein Information:	glutathione S-transferase Mu 5
UniProt Protein Name:	Glutathione S-transferase Mu 5
UniProt Synonym Protein Names:	GST class-mu 5; GSTM5-5
Protein Family:	Glutathione S-transferase
UniProt Gene Name:	GSTM5
UniProt Entry Name:	GSTM5_HUMAN

Western blot analysis of extracts of various cell lines, using GSTM5 antibody (CAB15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

Antibodies

GSTM5 Antibody (PACO09618)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)