Metabolism Antibodies 1
Anti-GSS Antibody (CAB1069)
- SKU:
- CAB1069
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-GSS Antibody |
| Antibody SKU: | CAB1069 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-474 of human GSS (NP_000169.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | DU145, SW480, K-562, BT-474, 22Rv1, Raji, Mouse kidney, Mouse liver, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-474 of human GSS (NP_000169.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATN WGSL LQDK QQLE ELAR QAVD RALA EGVL LRTS QEPT SSEV VSYA PFTL FPSL VPSA LLEQ AYAV QMDF NLLV DAVS QNAA FLEQ TLSS TIKQ DDFT ARLF DIHK QVLK EGIA QTVF LGLN RSDY MFQR SADG SPAL KQIE INTI SASF GGLA SRTP AVHR HVLS VLSK TKEA GKIL SNNP SKGL ALGI AKAW ELYG SPNA LVLL IAQE KERN IFDQ RAIE NELL ARNI HVIR RTFE DISE KGSL DQDR RLFV DGQE IAVV YFRD GYMP RQYS LQNW EARL LLER SHAA KCPD IATQ LAGT KKVQ QELS RPGM LEML LPGQ PEAV ARLR ATFA GLYS LDVG EEGD QAIA EALA APSR FVLK PQRE GGGN NLYG EEMV QALK QLKD SEER ASYI LMEK IEPE PFEN CLLR PGSP ARVV QCIS ELGI FGVY VRQE KTLV MNKH VGHL LRTK AIEH ADGG VAAG VAVL DNPY PV |
| Gene ID: | 2937 |
| Uniprot: | P48637 |
| Cellular Location: | |
| Calculated MW: | 40kDa/52kDa |
| Observed MW: | 53kDa |
| Synonyms: | GSS, GSHS, HEL-S-64p, HEL-S-88n |
| Background: | Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. |
| UniProt Protein Function: | GSS: Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5- oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. Belongs to the eukaryotic GSH synthase family. |
| UniProt Protein Details: | Protein type:EC 6.3.2.3; Other Amino Acids Metabolism - glutathione; Ligase Chromosomal Location of Human Ortholog: 20q11.2 Cellular Component: cytosol Molecular Function:protein homodimerization activity; glutathione binding; glycine binding; magnesium ion binding; glutathione synthase activity; ATP binding Biological Process: amino acid metabolic process; response to nutrient levels; nervous system development; response to cadmium ion; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; response to amino acid stimulus; aging Disease: Glutathione Synthetase Deficiency; Glutathione Synthetase Deficiency Of Erythrocytes, Hemolytic Anemia Due To |
| NCBI Summary: | Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P48637 |
| NCBI GenInfo Identifier: | 1346191 |
| NCBI Gene ID: | 2937 |
| NCBI Accession: | P48637.1 |
| UniProt Secondary Accession: | P48637,Q4TTD9, B2R697, B6F210, E1P5P9, |
| UniProt Related Accession: | P48637 |
| Molecular Weight: | 474 |
| NCBI Full Name: | Glutathione synthetase |
| NCBI Synonym Full Names: | glutathione synthetase |
| NCBI Official Symbol: | GSS |
| NCBI Official Synonym Symbols: | GSHS; HEL-S-64p; HEL-S-88n |
| NCBI Protein Information: | glutathione synthetase; GSH-S; GSH synthetase; glutathione synthase; epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n |
| UniProt Protein Name: | Glutathione synthetase |
| UniProt Synonym Protein Names: | Glutathione synthase |
| Protein Family: | Glutathione synthetase |
| UniProt Gene Name: | GSS |
| UniProt Entry Name: | GSHB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GSS antibody (CAB1069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HeLa cells using GSS antibody (CAB1069). Blue: DAPI for nuclear staining. |
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