Epigenetics & Nuclear Signaling Antibodies 2
Anti-GSPT1 Antibody (CAB16214)
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- SKU:
- CAB16214
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-GSPT1 Antibody |
| Antibody SKU: | CAB16214 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 138-420 of human GSPT1 (NP_001123478.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A549, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 138-420 of human GSPT1 (NP_001123478.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SMEL SEPI ENGE TEMS PEES WEHK EEIS EAEP GGGS LGDG RPPE ESAH EMME EEEE IPKP KSVV APPG APKK EHVN VVFI GHVD AGKS TIGG QIMY LTGM VDKR TLEK YERE AKEK NRET WYLS WALD TNQE ERDK GKTV EVGR AYFE TEKK HFTI LDAP GHKS FVPN MIGG ASQA DLAV LVIS ARKG EFET GFEK GGQT REHA MLAK TAGV KHLI VLIN KMDD PTVN WSNE RYEE CKEK LVPF LKKV GFNP KKDI HFMP CSGL TGAN LKEQ SDFC PWYI GLP |
| Gene ID: | 2935 |
| Uniprot: | P15170 |
| Cellular Location: | |
| Calculated MW: | 55kDa/68kDa |
| Observed MW: | 80kDa |
| Synonyms: | GSPT1, 551G9.2, ETF3A, GST1, eRF3a |
| Background: |
| UniProt Protein Function: | Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. |
| UniProt Code: | P15170 |
| NCBI GenInfo Identifier: | 121688 |
| NCBI Gene ID: | 2935 |
| NCBI Accession: | P15170.1 |
| UniProt Secondary Accession: | P15170,Q96GF2, J3KQG6, |
| UniProt Related Accession: | P15170 |
| Molecular Weight: | 68,700 Da |
| NCBI Full Name: | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A |
| NCBI Synonym Full Names: | G1 to S phase transition 1 |
| NCBI Official Symbol: | GSPT1 |
| NCBI Official Synonym Symbols: | GST1; ETF3A; eRF3a; 551G9.2 |
| NCBI Protein Information: | eukaryotic peptide chain release factor GTP-binding subunit ERF3A |
| UniProt Protein Name: | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A |
| UniProt Synonym Protein Names: | G1 to S phase transition protein 1 homolog |
| Protein Family: | Eukaryotic peptide chain release factor GTP-binding |
| UniProt Gene Name: | GSPT1 |
| UniProt Entry Name: | ERF3A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GSPT1 antibody (CAB16214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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