Metabolism Antibodies 1

Anti-GSK3Alpha Antibody (CAB0645)

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SKU:
CAB0645
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-GSK3Alpha Antibody
Antibody SKU:CAB0645
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 409-483 of human GSK3Alpha (NP_063937.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:1000 IF 1:20 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-431, HeLa, B-cell, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 409-483 of human GSK3Alpha (NP_063937.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LGTQ LPNN RPLP PLFN FSAG ELSI QPSL NAIL IPPH LRSP AGTT TLTP SSQA LTET PTSS DWQS TDAT PTLT NSS
Gene ID:2931
Uniprot:P49840
Cellular Location:
Calculated MW:50kDa
Observed MW:51kDa
Synonyms:GSK3 alpha, GSK3A
Background:This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
UniProt Protein Function:GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418
UniProt Protein Details:

Protein type:EC 2.7.11.26; EC 2.7.11.1; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: beta-catenin destruction complex; cytosol

Molecular Function:protein binding; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: cellular response to insulin stimulus; insulin receptor signaling pathway; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of TOR signaling pathway; positive regulation of cAMP biosynthetic process; positive regulation of heart contraction; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of systemic arterial blood pressure

NCBI Summary:This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]
UniProt Code:P49840
NCBI GenInfo Identifier:12644292
NCBI Gene ID:2931
NCBI Accession:P49840.2
UniProt Secondary Accession:P49840,O14959,
UniProt Related Accession:P49840
Molecular Weight:50,981 Da
NCBI Full Name:Glycogen synthase kinase-3 alpha
NCBI Synonym Full Names:glycogen synthase kinase 3 alpha
NCBI Official Symbol:GSK3A  
NCBI Protein Information:glycogen synthase kinase-3 alpha
UniProt Protein Name:Glycogen synthase kinase-3 alpha
UniProt Synonym Protein Names:Serine/threonine-protein kinase GSK3A (EC:2.7.11.1)
Protein Family:Glycogen synthase kinase
UniProt Gene Name:GSK3A  
UniProt Entry Name:GSK3A_HUMAN
Anti-GSK3Alpha Antibody (CAB0645)Western blot analysis of extracts of various cell lines, using GSK3a antibody (CAB0645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-GSK3Alpha Antibody (CAB0645)Immunofluorescence analysis of NIH/3T3 cells using GSK3a antibody (CAB0645) at dilution of 1:100. Blue: DAPI for nuclear staining.
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