Immunology Antibodies 2

Anti-GSDMD (Full Length+C terminal) Antibody (CAB17308)

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SKU:
CAB17308
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-GSDMD (Full Length+C terminal) Antibody
Antibody SKU:CAB17308
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human GSDMD (NP_079012.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:PC-3, THP-1, RAW264.7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human GSDMD (NP_079012.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:79792
Uniprot:P57764
Cellular Location:
Calculated MW:53kDa
Observed MW:53KDa/23KDa
Synonyms:GSDMD, DF5L, DFNA5L, FKSG10, GSDMDC1
Background:Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
UniProt Protein Function:FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
UniProt Protein Details:

Protein type:Lipid-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol; extracellular region; extracellular space; ficolin-1-rich granule lumen; NLRP3 inflammasome complex; nucleoplasm; plasma membrane; tertiary granule lumen

Molecular Function:cardiolipin binding; phosphatidic acid binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-4-phosphate binding; phosphatidylserine binding; protein binding

Biological Process: cellular response to extracellular stimulus; cytolysis; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; inflammatory response; innate immune response; neutrophil degranulation; pore complex assembly; positive regulation of interleukin-1 beta secretion; protein homooligomerization; pyroptosis

NCBI Summary:Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
UniProt Code:P57764
NCBI GenInfo Identifier:261878494
NCBI Gene ID:79792
NCBI Accession:NP_001159709.1
UniProt Secondary Accession:P57764,Q96Q98, A8K702, D3DWJ9,
UniProt Related Accession:P57764
Molecular Weight:52,801 Da
NCBI Full Name:gasdermin-D
NCBI Synonym Full Names:gasdermin D
NCBI Official Symbol:GSDMD  
NCBI Official Synonym Symbols:DF5L; DFNA5L; FKSG10; GSDMDC1  
NCBI Protein Information:gasdermin-D
UniProt Protein Name:Gasdermin-D
UniProt Synonym Protein Names:Gasdermin domain-containing protein 1
Protein Family:Gasdermin
UniProt Gene Name:GSDMD  
Anti-GSDMD (Full Length+C terminal) Antibody (CAB17308)Western blot analysis of extracts of various cell lines, using GSDMD antibody (CAB17308) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37'C for overnight and LPS (1 ug/ml) at 37'C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-GSDMD (Full Length+C terminal) Antibody (CAB17308)Western blot analysis of extracts of RAW264.7 cells, using GSDMD antibody (CAB17308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
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