Metabolism Antibodies 2
Anti-GRK6 Antibody (CAB6379)
- SKU:
- CAB6379
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-GRK6 Antibody |
| Antibody SKU: | CAB6379 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human GRK6 (NP_001004106.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, Jurkat, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human GRK6 (NP_001004106.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MELE NIVA NTVL LKAR EGGG GNRK GKSK KWRQ MLQF PHIS QCEE LRLS LERD YHSL CERQ PIGR LLFR EFCA TRPE LSRC VAFL DGVA EYEV TPDD KRKA CGRQ LTQN FLSH TGPD LIPE VPRQ LVTN CTQR LEQG PCKD LFQE LTRL THEY LSVA PFAD YLDS IYFN RF |
| Gene ID: | 2870 |
| Uniprot: | P43250 |
| Cellular Location: | Lipid-anchor, Membrane |
| Calculated MW: | 64kDa/65kDa/67kDa |
| Observed MW: | 66kDa |
| Synonyms: | GRK6, GPRK6 |
| Background: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. |
| UniProt Protein Function: | GRK6: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamin receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro). Widely expressed. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.11.16; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; GRK subfamily Chromosomal Location of Human Ortholog: 5q35 Cellular Component: membrane Molecular Function:G-protein coupled receptor kinase activity; protein binding; ATP binding Biological Process: Wnt receptor signaling pathway; protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway |
| NCBI Summary: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P43250 |
| NCBI GenInfo Identifier: | 20141386 |
| NCBI Gene ID: | 2870 |
| NCBI Accession: | P43250.2 |
| UniProt Secondary Accession: | P43250,O60541, Q13652, |
| UniProt Related Accession: | P43250 |
| Molecular Weight: | 64,294 Da |
| NCBI Full Name: | G protein-coupled receptor kinase 6 |
| NCBI Synonym Full Names: | G protein-coupled receptor kinase 6 |
| NCBI Official Symbol: | GRK6 |
| NCBI Official Synonym Symbols: | GPRK6 |
| NCBI Protein Information: | G protein-coupled receptor kinase 6 |
| UniProt Protein Name: | G protein-coupled receptor kinase 6 |
| UniProt Synonym Protein Names: | G protein-coupled receptor kinase GRK6 |
| Protein Family: | G protein-coupled receptor kinase |
| UniProt Gene Name: | GRK6 |
| UniProt Entry Name: | GRK6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GRK6 antibody (CAB6379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of MCF7 cells using GRK6 antibody (CAB6379). Blue: DAPI for nuclear staining. |
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