Signal Transduction Antibodies 2
Anti-GRK1 Antibody (CAB2966)
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- SKU:
- CAB2966
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-GRK1 Antibody |
| Antibody SKU: | CAB2966 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse eye, Rat liver, Rat eye |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDFG SLET VVAN SAFI AARG SFDG SSSQ PSRD KKYL AKLK LPPL SKCE SLRD SLSL EFES VCLE QPIG KKLF QQFL QSAE KHLP ALEL WKDI EDYD TADN DLQP QKAQ TILA QYLD PQAK LFCS FLDE GIVA KFKE GPVE IQDG LFQP LLQA TLAH LGQA |
| Gene ID: | 6011 |
| Uniprot: | Q15835 |
| Cellular Location: | Lipid-anchor, Membrane |
| Calculated MW: | 63kDa |
| Observed MW: | 64kDa |
| Synonyms: | GRK1, GPRK1, RHOK, RK |
| Background: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). |
| UniProt Protein Function: | GRK1: a Ser/Thr of the GRK family. Phosphorylates and inactivates photon-activated rhodopsin. LOF mutations lead to type 2 Oguchi disease, a form of night blindness. A link between RHOK and retinitis pigmentosa is now thought to be unlikely |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.14; Kinase, protein; AGC group; GRK family; GRK subfamily Chromosomal Location of Human Ortholog: 13q34 Molecular Function:G-protein coupled receptor kinase activity; rhodopsin kinase activity; ATP binding; protein kinase activity Biological Process: rhodopsin mediated signaling; post-embryonic retina morphogenesis in camera-type eye; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; protein amino acid autophosphorylation; photoreceptor cell morphogenesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of phosphorylation; negative regulation of apoptosis Disease: Oguchi Disease 2 |
| NCBI Summary: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15835 |
| NCBI GenInfo Identifier: | 2833269 |
| NCBI Gene ID: | 6011 |
| NCBI Accession: | Q15835.1 |
| UniProt Secondary Accession: | Q15835,Q53X14, |
| UniProt Related Accession: | Q15835 |
| Molecular Weight: | 563 |
| NCBI Full Name: | Rhodopsin kinase |
| NCBI Synonym Full Names: | G protein-coupled receptor kinase 1 |
| NCBI Official Symbol: | GRK1 |
| NCBI Official Synonym Symbols: | RK; RHOK; GPRK1 |
| NCBI Protein Information: | rhodopsin kinase |
| UniProt Protein Name: | Rhodopsin kinase |
| UniProt Synonym Protein Names: | G protein-coupled receptor kinase 1 |
| UniProt Gene Name: | GRK1 |
| UniProt Entry Name: | RK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GRK1 antibody (CAB2966) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat retina using GRK1 antibody (CAB2966) at dilution of 1:200 (40x lens). |
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