Antibody Name:	Anti-GRIK2 Antibody
Antibody SKU:	CAB1939
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-300 of human GRIK2 (NP_068775.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-300 of human GRIK2 (NP_068775.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QGTT HVLR FGGI FEYV ESGP MGAE ELAF RFAV NTIN RNRT LLPN TTLT YDTQ KINL YDSF EASK KACD QLSL GVAA IFGP SHSS SANA VQSI CNAL GVPH IQTR WKHQ VSDN KDSF YVSL YPDF SSLS RAIL DLVQ FFKW KTVT VVYD DSTG LIRL QELI KAPS RYNL RLKI RQLP ADTK DAKP LLKE MKRG KEFH VIFD CSHE MAAG ILKQ ALAM GMMT EYYH YIFT TLDL FALD VEPY RYSG VNMT GFRI LNTE NTQV SSII EKWS MER
Gene ID:	2898
Uniprot:	Q13002
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	66kDa/77kDa/93kDa/97kDa/100kDa/102kDa
Observed MW:	115kDa

Synonyms:

GRIK2, EAA4, GLR6, GLUK6, GLUR6, GluK2, MRT6, kainate 2

Background:

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive mental retardation.

UniProt Protein Function:	GluR6: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2. Defects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6). It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Channel, calcium; Channel, ligand-gated; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 6q16.3 Cellular Component: dendrite cytoplasm; presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; dendrite; postsynaptic density; plasma membrane; terminal button; perikaryon; cell junction Molecular Function:protein homodimerization activity; kainate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; PDZ domain binding Biological Process: regulation of synaptic transmission; regulation of long-term neuronal synaptic plasticity; synaptic transmission, glutamatergic; behavioral fear response; glutamate signaling pathway; generation of action potential; negative regulation of synaptic transmission, glutamatergic; regulation of short-term neuronal synaptic plasticity; regulation of JNK cascade; receptor clustering; positive regulation of synaptic transmission; cellular calcium ion homeostasis; synaptic transmission; intracellular protein transport; regulation of inhibitory postsynaptic membrane potential; neuron apoptosis; ionotropic glutamate receptor signaling pathway; transport; positive regulation of neuron apoptosis; negative regulation of neuron apoptosis; regulation of excitatory postsynaptic membrane potential Disease: Mental Retardation, Autosomal Recessive 6
NCBI Summary:	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive mental retardation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13002
NCBI GenInfo Identifier:	2492627
NCBI Gene ID:	2898
NCBI Accession:	Q13002.1
UniProt Secondary Accession:	Q13002,Q8WWS1, Q96KS6, Q96KS7, Q96KS8, A6NMY9, B5MCV0 D7RWZ3, D7RWZ4, D7RWZ5, D7RWZ6, D7RWZ7,
UniProt Related Accession:	Q13002
Molecular Weight:	908
NCBI Full Name:	Glutamate receptor ionotropic, kainate 2
NCBI Synonym Full Names:	glutamate receptor, ionotropic, kainate 2
NCBI Official Symbol:	GRIK2
NCBI Official Synonym Symbols:	EAA4; GLR6; MRT6; GLUK6; GLUR6; GluK2
NCBI Protein Information:	glutamate receptor ionotropic, kainate 2; gluR-6; bA487F5.1; glutamate receptor 6; glutamate receptor form A; glutamate receptor form B; glutamate receptor form C; glutamate receptor form D; glutamate receptor form E; excitatory amino acid receptor 4
UniProt Protein Name:	Glutamate receptor ionotropic, kainate 2
UniProt Synonym Protein Names:	Excitatory amino acid receptor 4; EAA4; Glutamate receptor 6; GluR-6; GluR6
Protein Family:	Glutamate receptor ionotropic
UniProt Gene Name:	GRIK2
UniProt Entry Name:	GRIK2_HUMAN

	Western blot analysis of extracts of various cell lines, using GRIK2 antibody (CAB1939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using GRIK2 antibody (CAB1939). Blue: DAPI for nuclear staining.