Epigenetics & Nuclear Signaling Antibodies 1

Anti-GRHL2 Antibody (CAB10153)

(No reviews yet) Write a Review
SKU:
CAB10153
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-GRHL2 Antibody
Antibody SKU:CAB10153
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-250 of human GRHL2 (NP_079191.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:SH-SY5Y
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-250 of human GRHL2 (NP_079191.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LSVS KASD SQED QEKR NCLG TSEA QSNL SGGE NRVQ VLKT VPVN LSLN QDHL ENSK REQY SISF PESS AIIP VSGI TVVK AEDF TPVF MAPP VHYP RGDG EEQR VVIF EQTQ YDVP SLAT HSAY LKDD QRST PDST YSES FKDA ATEK FRSA SVGA EEYM YDQT SSGT FQY
Gene ID:79977
Uniprot:Q6ISB3
Cellular Location:Membrane, Nucleus
Calculated MW:69kDa/71kDa
Observed MW:75kDa
Synonyms:GRHL2, BOM, DFNA28, ECTDS, TFCP2L3
Background:The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).
UniProt Protein Function:GRHL2: May function as a transcription factor. Defects in GRHL2 are the cause of deafness autosomal dominant type 28 (DFNA28). DFNA28 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA28 is characterized by mild to moderate hearing loss across most frequencies that progressed to severe loss in the higher frequencies by the fifth decade. Age at onset varied, with the earliest case documented at 7 years of age. Belongs to the grh/CP2 family. Grainyhead subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 8q22.3

Cellular Component: intercellular junction; nucleoplasm; nucleus; plasma membrane

Molecular Function:chromatin DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: brain development; cell adhesion; cell proliferation; epidermis development; negative regulation of keratinocyte differentiation; neural tube closure; neural tube development; positive regulation of telomerase activity; positive regulation of transcription from RNA polymerase II promoter

Disease: Deafness, Autosomal Dominant 28; Ectodermal Dysplasia/short Stature Syndrome

NCBI Summary:The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).[provided by RefSeq, Mar 2009]
UniProt Code:Q6ISB3
NCBI GenInfo Identifier:74736618
NCBI Gene ID:79977
NCBI Accession:Q6ISB3.1
UniProt Secondary Accession:Q6ISB3,Q6NT03, Q9H8B8, A1L303,
UniProt Related Accession:Q6ISB3
Molecular Weight:69,322 Da
NCBI Full Name:Grainyhead-like protein 2 homolog
NCBI Synonym Full Names:grainyhead like transcription factor 2
NCBI Official Symbol:GRHL2  
NCBI Official Synonym Symbols:BOM; ECTDS; DFNA28; TFCP2L3  
NCBI Protein Information:grainyhead-like protein 2 homolog
UniProt Protein Name:Grainyhead-like protein 2 homolog
UniProt Synonym Protein Names:Brother of mammalian grainyhead; Transcription factor CP2-like 3
Protein Family:Grainyhead-like protein
UniProt Gene Name:GRHL2  
UniProt Entry Name:GRHL2_HUMAN
Anti-GRHL2 Antibody (CAB10153)Western blot analysis of extracts of SH-SY5Y cells, using GRHL2 antibody (CAB10153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
View AllClose

0 Reviews

View AllClose

Related Products

GRHL2 Polyclonal Antibody

GRHL2 Polyclonal Antibody

OverviewProduct Name:GRHL2 Polyclonal AntibodyProduct Code:CAB24468Reactivity:Human, RatApplications:Western blotting