Name: Anti-GRB7 Antibody (CAB5690)
Brand: Cell Biology Antibodies 9
SKU: CAB5690
Availability: OutOfStock

Antibody Name:	Anti-GRB7 Antibody
Antibody SKU:	CAB5690
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human GRB7 (NP_001229372.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, 22Rv1, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human GRB7 (NP_001229372.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MELD LSPP HLSS SPED LCPA PGTP PGTP RPPD TPLP EEVK RSQP LLIP TTGR KLRE EERR ATSL PSIP NPFP ELCS PPSQ SPIL GGPS SARG LLPR DASR PHVV KVYS EDGA CRSV EVAA GATA RHVC EMLV QRAH ALSD
Gene ID:	2886
Uniprot:	Q14451
Cellular Location:	Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Peripheral membrane protein, focal adhesion
Calculated MW:	49kDa/55kDa/59kDa/62kDa
Observed MW:	60kDa

Synonyms:

GRB7

Background:

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	GRB7: Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress. Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1. Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Belongs to the GRB7/10/14 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 17q12 Cellular Component: cell projection; focal adhesion; stress granule; plasma membrane; cytosol Molecular Function:identical protein binding; protein binding; SH3/SH2 adaptor activity; RNA binding; protein kinase binding; phosphoinositide binding Biological Process: epidermal growth factor receptor signaling pathway; stress granule assembly; positive regulation of signal transduction; negative regulation of translation; blood coagulation; leukocyte migration; positive regulation of cell migration
NCBI Summary:	The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
UniProt Code:	Q14451
NCBI GenInfo Identifier:	116242503
NCBI Gene ID:	2886
NCBI Accession:	Q14451.2
UniProt Secondary Accession:	Q14451,Q53YD3, Q92568, Q96DF9, Q9Y220, B2RAV1, B3KNL0 B3KWP9, B7WP75, J3KQM4,
UniProt Related Accession:	Q14451
Molecular Weight:	532
NCBI Full Name:	Growth factor receptor-bound protein 7
NCBI Synonym Full Names:	growth factor receptor-bound protein 7
NCBI Official Symbol:	GRB7
NCBI Protein Information:	growth factor receptor-bound protein 7; B47; GRB7 adapter protein; epidermal growth factor receptor GRB-7
UniProt Protein Name:	Growth factor receptor-bound protein 7
UniProt Synonym Protein Names:	B47; Epidermal growth factor receptor GRB-7; GRB7 adapter protein
Protein Family:	Growth factor receptor-bound protein
UniProt Gene Name:	GRB7
UniProt Entry Name:	GRB7_HUMAN

Western blot analysis of extracts of various cell lines, using GRB7 antibody (CAB5690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

GRB7 Antibody (PACO09585)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)