Immunology Antibodies 2
Anti-GRB2 Antibody (CAB5689)
- SKU:
- CAB5689
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-GRB2 Antibody |
| Antibody SKU: | CAB5689 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRB2 (NP_002077.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SW620, Raji, BT-474, HepG2, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRB2 (NP_002077.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEAI AKYD FKAT ADDE LSFK RGDI LKVL NEEC DQNW YKAE LNGK DGFI PKNY IEMK PHPW FFGK IPRA KAEE MLSK QRHD GAFL IRES ESAP GDFS LSVK FGND VQHF KVLR DGAG KYFL WVVK FNSL NELV DYHR STSV SRNQ QIFL RDIE QVPQ QPTY VQAL FDFD PQED GELG FRRG DFIH VMDN SDPN WWKG ACHG QTGM FPRN YVTP VNRN V |
| Gene ID: | 2885 |
| Uniprot: | P62993 |
| Cellular Location: | Cytoplasm, Endosome, Golgi apparatus, Nucleus |
| Calculated MW: | 20kDa/25kDa |
| Observed MW: | 33kDa |
| Synonyms: | ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2, GRB2 |
| Background: | The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. |
| NCBI Summary: | The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62993 |
| NCBI GenInfo Identifier: | 51702266 |
| NCBI Gene ID: | 2885 |
| NCBI Accession: | P62993.1 |
| UniProt Secondary Accession: | P62993,P29354, Q14450, Q63057, Q63059, |
| UniProt Related Accession: | P62993 |
| Molecular Weight: | 20,557 Da |
| NCBI Full Name: | Growth factor receptor-bound protein 2 |
| NCBI Synonym Full Names: | growth factor receptor bound protein 2 |
| NCBI Official Symbol: | GRB2 |
| NCBI Official Synonym Symbols: | ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2 |
| NCBI Protein Information: | growth factor receptor-bound protein 2 |
| UniProt Protein Name: | Growth factor receptor-bound protein 2 |
| UniProt Synonym Protein Names: | Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2 |
| Protein Family: | Growth factor receptor-bound protein |
| UniProt Gene Name: | GRB2 |
| UniProt Entry Name: | GRB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GRB2 antibody (CAB5689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using GRB2 antibody (CAB5689) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using GRB2 antibody (CAB5689). Blue: DAPI for nuclear staining. |
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