Name: Anti-GRB14 Antibody (CAB2742)
Brand: Cell Biology Antibodies 8
SKU: CAB2742
Availability: OutOfStock

Antibody Name:	Anti-GRB14 Antibody
Antibody SKU:	CAB2742
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human GRB14

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:25 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Human fetal intestine

Immunogen:	Recombinant protein of human GRB14
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	2888
Uniprot:	Q14449
Cellular Location:	Cytoplasm, Endosome membrane, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:	51kDa/60kDa
Observed MW:	61kDa

Synonyms:

GRB14

Background:

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	GRB14: Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. Belongs to the GRB7/10/14 family.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 2q22-q24 Cellular Component: Golgi membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; endosome membrane; cytosol Molecular Function:SH3/SH2 adaptor activity Biological Process: positive regulation of signal transduction; signal transduction; blood coagulation; leukocyte migration
NCBI Summary:	The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:	Q14449
NCBI GenInfo Identifier:	59802920
NCBI Gene ID:	2888
NCBI Accession:	Q14449.2
UniProt Secondary Accession:	Q14449,Q7Z6I1, B7Z7F9,
UniProt Related Accession:	Q14449
Molecular Weight:	540
NCBI Full Name:	Growth factor receptor-bound protein 14
NCBI Synonym Full Names:	growth factor receptor-bound protein 14
NCBI Official Symbol:	GRB14
NCBI Protein Information:	growth factor receptor-bound protein 14; GRB14 adapter protein
UniProt Protein Name:	Growth factor receptor-bound protein 14
UniProt Synonym Protein Names:	GRB14 adapter protein
Protein Family:	Growth factor receptor-bound protein
UniProt Gene Name:	GRB14
UniProt Entry Name:	GRB14_HUMAN

Western blot analysis of extracts of human fetal intestine, using GRB14 antibody (CAB2742). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies

GRB14 Antibody (PACO00884)

GRB14 Antibody (PACO09583)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)